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The opossum kidney cell type IIa Na/P(i) cotransporter is a phosphoprotein


Jankowski, M; Hilfiker, H; Biber, J; Murer, H (2001). The opossum kidney cell type IIa Na/P(i) cotransporter is a phosphoprotein. Kidney & Blood Pressure Research, 24(1):1-4.

Abstract

BACKGROUND/AIM: Parathyroid hormone (PTH)-dependent inhibition of proximal tubular P(i) reabsorption is mediated by protein kinase A and/or C and is associated with reduced border membrane expression of the type IIa Na/P(i) cotransporter. The aim of this study was to analyze phosphorylation of the type IIa cotransporter protein. METHODS: Opossum kidney cells were used as a 'proximal tubular' cell model. Protein phosphorylation was determined by immunoprecipitation of the type IIa Na/P(i) cotransporter, followed by autoradiography. The transporter protein content was evaluated by Western blotting and transport activity by tracer P(i) uptake. RESULTS: Under control conditions (no PTH) the transporter was phosphorylated; upon treatment with PTH, a decrease in phosphorylation was observed. A protein phosphatase inhibitor (okadaic acid) was unable to prevent PTH-induced Na/P(i) cotransporter inhibition but reduced transporter degradation. CONCLUSION: The type IIa Na/P(i) cotransporter is a phosphoprotein, but alterations in its phosphorylation seem not to be involved in P(i) transport inhibition.

Abstract

BACKGROUND/AIM: Parathyroid hormone (PTH)-dependent inhibition of proximal tubular P(i) reabsorption is mediated by protein kinase A and/or C and is associated with reduced border membrane expression of the type IIa Na/P(i) cotransporter. The aim of this study was to analyze phosphorylation of the type IIa cotransporter protein. METHODS: Opossum kidney cells were used as a 'proximal tubular' cell model. Protein phosphorylation was determined by immunoprecipitation of the type IIa Na/P(i) cotransporter, followed by autoradiography. The transporter protein content was evaluated by Western blotting and transport activity by tracer P(i) uptake. RESULTS: Under control conditions (no PTH) the transporter was phosphorylated; upon treatment with PTH, a decrease in phosphorylation was observed. A protein phosphatase inhibitor (okadaic acid) was unable to prevent PTH-induced Na/P(i) cotransporter inhibition but reduced transporter degradation. CONCLUSION: The type IIa Na/P(i) cotransporter is a phosphoprotein, but alterations in its phosphorylation seem not to be involved in P(i) transport inhibition.

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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2001
Deposited On:11 Feb 2008 12:23
Last Modified:29 Aug 2017 15:12
Publisher:Karger
ISSN:1420-4096
Additional Information:The final, published version of this article is available at http://www.karger.com/?doi=10.1159/000054198
Publisher DOI:https://doi.org/10.1159/000054198
PubMed ID:11173999

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