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Ecophysiological differences of betaproteobacterial populations in two hydrochemically distinct compartments of a subtropical lagoon


Alonso, C; Zeder, M; Piccini, C; Conde, D; Pernthaler, J (2009). Ecophysiological differences of betaproteobacterial populations in two hydrochemically distinct compartments of a subtropical lagoon. Environmental Microbiology, 11(4):867-876.

Abstract

We studied the population sizes and substrate incorporation
patterns of three phylogenetic groups of
Betaproteobacteria in a coastal subtropical lagoon
that is characterized by a sharp transition from humic
freshwater to turbid brackish water. Various cellular
processes were addressed by short-term incubations
with four radiolabelled compounds and microautoradiographic
assessment of substrate incorporation.
Group-specific differences in the abundances and the
respective physiological state of the three populations
were observed upon transfer from the humicrich
compartment to the main body of the lagoon
(estimated at 1–2 days). Members of the clade B of
Polynucleobacter (PnecB) experienced only an insignificant
change in cell numbers, but displayed a
general metabolic downshift, carbon metabolism
(glucose incorporation) being most affected. By contrast,
bacteria from the closely related Polynucleobacter
C clade (PnecC) clearly differed in total
abundances and in the numbers of DNA-synthesizing
or glucose incorporating cells. At the same time,
PnecC bacteria maintained comparable levels of
protein synthesis (leucine uptake) in both lagoon
compartments, and the proportion of cells incorporating
N-acetylglucosamine was even higher in the main
body of the lagoon. Members of the R-BT lineage
showed little changes in cell numbers, DNA synthesis
and carbon metabolism. Altogether, the observed patterns
of substrate metabolism suggest that different
bacterial populations in the lagoon undergo specific
physiological adjustments in response to changing
environmental conditions.

Abstract

We studied the population sizes and substrate incorporation
patterns of three phylogenetic groups of
Betaproteobacteria in a coastal subtropical lagoon
that is characterized by a sharp transition from humic
freshwater to turbid brackish water. Various cellular
processes were addressed by short-term incubations
with four radiolabelled compounds and microautoradiographic
assessment of substrate incorporation.
Group-specific differences in the abundances and the
respective physiological state of the three populations
were observed upon transfer from the humicrich
compartment to the main body of the lagoon
(estimated at 1–2 days). Members of the clade B of
Polynucleobacter (PnecB) experienced only an insignificant
change in cell numbers, but displayed a
general metabolic downshift, carbon metabolism
(glucose incorporation) being most affected. By contrast,
bacteria from the closely related Polynucleobacter
C clade (PnecC) clearly differed in total
abundances and in the numbers of DNA-synthesizing
or glucose incorporating cells. At the same time,
PnecC bacteria maintained comparable levels of
protein synthesis (leucine uptake) in both lagoon
compartments, and the proportion of cells incorporating
N-acetylglucosamine was even higher in the main
body of the lagoon. Members of the R-BT lineage
showed little changes in cell numbers, DNA synthesis
and carbon metabolism. Altogether, the observed patterns
of substrate metabolism suggest that different
bacterial populations in the lagoon undergo specific
physiological adjustments in response to changing
environmental conditions.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:580 Plants (Botany)
Language:English
Date:2009
Deposited On:19 Feb 2009 20:25
Last Modified:21 Nov 2017 14:00
Publisher:Wiley-Blackwell
ISSN:1462-2912
Publisher DOI:https://doi.org/10.1111/j.1462-2920.2008.01807.x

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