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The Applied Biosystems™ NGM Detect™ PCR Amplification Kit – As promising as promised?


Burch, S; Sulzer, A; Voegeli, P; Morf, N V; Gysi, M; Kratzer, A (2017). The Applied Biosystems™ NGM Detect™ PCR Amplification Kit – As promising as promised? Forensic Science International: Genetics Supplement Series, 6:e504-e506.

Abstract

The new Applied Biosystems™ NGM Detect™ PCR Amplification kit comes with several innovations compared to the NGM SElect™ kit, e.g. a six color approach and rearranged loci, which makes it an interesting candidate for analysis of forensic case work samples. The internal validation shows that the shorter amplification time and the higher input volume are some of its main advantages, whereas the higher allelic imbalance is its main disadvantage.Following validation a concordance study with 1320 samples described in a publication by Dion et al. [3] was executed. The results with NGM Detect™ were compared to the ones with NGM SElect™ and PowerPlex® ESI 17 and revealed eight discordances between NGM Detect™ and NGM SElect™ and only one between NGM Detect™ and PP ESI 17. The concordance rate between the STR kits was greater than 99.982 %.

Abstract

The new Applied Biosystems™ NGM Detect™ PCR Amplification kit comes with several innovations compared to the NGM SElect™ kit, e.g. a six color approach and rearranged loci, which makes it an interesting candidate for analysis of forensic case work samples. The internal validation shows that the shorter amplification time and the higher input volume are some of its main advantages, whereas the higher allelic imbalance is its main disadvantage.Following validation a concordance study with 1320 samples described in a publication by Dion et al. [3] was executed. The results with NGM Detect™ were compared to the ones with NGM SElect™ and PowerPlex® ESI 17 and revealed eight discordances between NGM Detect™ and NGM SElect™ and only one between NGM Detect™ and PP ESI 17. The concordance rate between the STR kits was greater than 99.982 %.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Legal Medicine
Dewey Decimal Classification:340 Law
610 Medicine & health
Language:English
Date:2017
Deposited On:19 Dec 2017 16:30
Last Modified:19 Feb 2018 09:47
Publisher:Elsevier
ISSN:1875-175X
OA Status:Closed
Publisher DOI:https://doi.org/10.1016/j.fsigss.2017.09.181

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