Header

UZH-Logo

Maintenance Infos

Rapid identification of rust resistance genes through cultivar-specific de novo chromosome assemblies


Thind, Anupriya K; Wicker, Thomas; Krattinger, Simon G (2017). Rapid identification of rust resistance genes through cultivar-specific de novo chromosome assemblies. In: Periyannan, Sam. Wheat Rust Diseases. New York, NY: Springer, 245-255.

Abstract

“Map-based cloning” is a frequently used approach to isolate rust resistance genes. A critical step during map-based cloning is the transition from genetic information, i.e., a genetic map, to physical sequence information. Bacterial artificial chromosome clones are often used to establish sequence information spanning a genetic interval. However, a major limitation of BAC clones consists in their small insert size of 100–200 kb. Targeted chromosome-based cloning via long-range assembly (TACCA) is a method that can replace BAC library screening. This approach involves chromosome flow-sorting and the establishment of a long-range de novo assembly. This chapter provides an overview of TACCA as well as a detailed description of sequence analyses, molecular marker development, and candidate gene identification.

Abstract

“Map-based cloning” is a frequently used approach to isolate rust resistance genes. A critical step during map-based cloning is the transition from genetic information, i.e., a genetic map, to physical sequence information. Bacterial artificial chromosome clones are often used to establish sequence information spanning a genetic interval. However, a major limitation of BAC clones consists in their small insert size of 100–200 kb. Targeted chromosome-based cloning via long-range assembly (TACCA) is a method that can replace BAC library screening. This approach involves chromosome flow-sorting and the establishment of a long-range de novo assembly. This chapter provides an overview of TACCA as well as a detailed description of sequence analyses, molecular marker development, and candidate gene identification.

Statistics

Citations

Altmetrics

Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:580 Plants (Botany)
Language:English
Date:31 August 2017
Deposited On:04 Jan 2018 18:38
Last Modified:19 Feb 2018 09:58
Publisher:Springer
Series Name:Methods in Molecular Biology
ISSN:1064-3745
ISBN:978-1-4939-7248-7
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-4939-7249-4_21
PubMed ID:28856656

Download

Full text not available from this repository.
View at publisher

Get full-text in a library