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Detection of IgE-reactive proteins in hydrolysed dog foods


Roitel, Olivier; Bonnard, Lionel; Stella, Alexandre; Schiltz, Odile; Maurice, Delphine; Douchin, Gael; Jacquenet, Sandrine; Favrot, Claude; Bihain, Bernard E; Couturier, Nicolas (2017). Detection of IgE-reactive proteins in hydrolysed dog foods. Veterinary Dermatology, 28(6):589-e143.

Abstract

BACKGROUND

Commercial hydrolysed diets are used for the diagnosis of food allergy in dogs. The cleaved parent proteins are presumed to be too small to elicit an allergic response by reacting with allergen-specific immunoglobin E (IgE).

OBJECTIVES

To evaluate three commercial hydrolysed dog diets for proteins.

ANIMALS

Sera were collected from dogs with suspected food allergy.

METHODS

Two batches of each hydrolysed diet were examined by electrophoresis and visualized by Coomassie blue, silver nitrate staining and IgE immunoblotting.

RESULTS

From two to five proteins, ranging from 21 to 67 kDa, were detected in all three diets evaluated. Circulating IgE antibodies targeting these proteins were detected by immunoblotting of dog sera. Six different carbohydrate proteins were identified by mass spectrometry; maize/potato granule-bound starch synthase-1, soybean glycinin, soybean β-conglycinin α chain, potato aspartic protease inhibitor, rice glutelin type B1 and soybean sucrose-binding protein. Four of these proteins have been described as allergens in humans.

CONCLUSIONS

Some commercial hydrolysed diets contain carbohydrate proteins. Some dogs have circulating IgE antibodies targeting these proteins. The clinical significance of these findings is unknown.

Abstract

BACKGROUND

Commercial hydrolysed diets are used for the diagnosis of food allergy in dogs. The cleaved parent proteins are presumed to be too small to elicit an allergic response by reacting with allergen-specific immunoglobin E (IgE).

OBJECTIVES

To evaluate three commercial hydrolysed dog diets for proteins.

ANIMALS

Sera were collected from dogs with suspected food allergy.

METHODS

Two batches of each hydrolysed diet were examined by electrophoresis and visualized by Coomassie blue, silver nitrate staining and IgE immunoblotting.

RESULTS

From two to five proteins, ranging from 21 to 67 kDa, were detected in all three diets evaluated. Circulating IgE antibodies targeting these proteins were detected by immunoblotting of dog sera. Six different carbohydrate proteins were identified by mass spectrometry; maize/potato granule-bound starch synthase-1, soybean glycinin, soybean β-conglycinin α chain, potato aspartic protease inhibitor, rice glutelin type B1 and soybean sucrose-binding protein. Four of these proteins have been described as allergens in humans.

CONCLUSIONS

Some commercial hydrolysed diets contain carbohydrate proteins. Some dogs have circulating IgE antibodies targeting these proteins. The clinical significance of these findings is unknown.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Department of Small Animals
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Language:English
Date:December 2017
Deposited On:23 Jan 2018 10:47
Last Modified:19 Feb 2018 09:59
Publisher:Wiley-Blackwell Publishing, Inc.
ISSN:0959-4493
OA Status:Closed
Publisher DOI:https://doi.org/10.1111/vde.12473
PubMed ID:28770578

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