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A guide to designing germline-dependent epigenetic inheritance experiments in mammals


Bohacek, Johannes; Mansuy, Isabelle M (2017). A guide to designing germline-dependent epigenetic inheritance experiments in mammals. Nature Methods, 14(3):243-249.

Abstract

Recent work has demonstrated that environmental factors experienced by parents can affect their offspring across multiple generations, and that such transgenerational transmission can depend on the germline. Causal evidence for the involvement of germ cells is rare, however, and the underlying molecular mechanisms remain poorly understood. Further, studies often employ varying methods in experimental design and data interpretation. We provide a critical analysis of these issues and suggest possible solutions and guidelines for improving study design and generating reproducible and high-quality data.

Abstract

Recent work has demonstrated that environmental factors experienced by parents can affect their offspring across multiple generations, and that such transgenerational transmission can depend on the germline. Causal evidence for the involvement of germ cells is rare, however, and the underlying molecular mechanisms remain poorly understood. Further, studies often employ varying methods in experimental design and data interpretation. We provide a critical analysis of these issues and suggest possible solutions and guidelines for improving study design and generating reproducible and high-quality data.

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Additional indexing

Item Type:Journal Article, refereed, further contribution
Communities & Collections:04 Faculty of Medicine > Brain Research Institute
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2017
Deposited On:09 Jan 2018 15:37
Last Modified:19 Feb 2018 10:05
Publisher:Nature Publishing Group
ISSN:1548-7091
OA Status:Closed
Publisher DOI:https://doi.org/10.1038/nmeth.4181
PubMed ID:28245210

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