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Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA binding analyses in arabidopsis


You, Wanhui; Pien, Stéphane; Grossniklaus, Ueli (2017). Chromatin Immunoprecipitation Protocol for Histone Modifications and Protein-DNA binding analyses in arabidopsis. In: Kovalchuk, I. Plant Epigenetics. Boston, MA: Springer, 1-13.

Abstract

Epigenetic control of plant development via histone modifications is involved in different processes ranging from embryonic development, vegetative development, flowering time control, floral organ development, to pollen tube growth. The identification of an increasing number of epigenetically regulated processes was greatly advanced by methods allowing the survey of genome-wide histone modifications and chromatin-protein interactions. However, genome-wide approaches are too broad to access in detail a large number of histone modifications taking place at a single locus. Here, we provide a robust chromatin immunoprecipitation (ChIP) protocol, allowing in vivo analyses of multiple chromatin modifications and binding of histone modifiers in different plant organs and tissues. This method is quantitative and provides a way to study the dynamic state of chromatin during plant development and also in response to different environmental stimuli.

Abstract

Epigenetic control of plant development via histone modifications is involved in different processes ranging from embryonic development, vegetative development, flowering time control, floral organ development, to pollen tube growth. The identification of an increasing number of epigenetically regulated processes was greatly advanced by methods allowing the survey of genome-wide histone modifications and chromatin-protein interactions. However, genome-wide approaches are too broad to access in detail a large number of histone modifications taking place at a single locus. Here, we provide a robust chromatin immunoprecipitation (ChIP) protocol, allowing in vivo analyses of multiple chromatin modifications and binding of histone modifiers in different plant organs and tissues. This method is quantitative and provides a way to study the dynamic state of chromatin during plant development and also in response to different environmental stimuli.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Language:English
Date:2017
Deposited On:10 Jan 2018 15:24
Last Modified:19 Feb 2018 10:13
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:1456
ISSN:1064-3745
ISBN:978-1-4899-7706-9
OA Status:Closed
Publisher DOI:https://doi.org/10.1007/978-1-4899-7708-3_1
PubMed ID:27770353

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