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Identification of parent-of-origin-dependent QTLs using bulk-segregant sequencing (Bulk-Seq)


Pires, Nuno D; Grossniklaus, Ueli (2017). Identification of parent-of-origin-dependent QTLs using bulk-segregant sequencing (Bulk-Seq). In: Bemer, Marian; Baroux, Célia. Plant Chromatin Dynamics. New York: Springer, 361-371.

Abstract

Parent-of-origin effects play important roles in plant reproduction and are often mediated by epigenetic modifications at the histone or DNA level. However, the genetic basis underlying these modifications can be challenging to identify. Here, we describe an approach (Bulk-Seq) that can be used to map loci mediating parent-of-origin-dependent effects using whole-genome sequencing of pools of DNA.

Abstract

Parent-of-origin effects play important roles in plant reproduction and are often mediated by epigenetic modifications at the histone or DNA level. However, the genetic basis underlying these modifications can be challenging to identify. Here, we describe an approach (Bulk-Seq) that can be used to map loci mediating parent-of-origin-dependent effects using whole-genome sequencing of pools of DNA.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:580 Plants (Botany)
Language:English
Date:2017
Deposited On:10 Jan 2018 15:20
Last Modified:10 Jan 2018 15:20
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:1675
ISSN:1064-3745
ISBN:978-1-4939-7317-0
Publisher DOI:https://doi.org/10.1007/978-1-4939-7318-7_21
PubMed ID:29052202

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