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Automated computer-assisted quantitative analysis of intact murine lungs at the alveolar scale


Lovric, Goran; Vogiatzis Oikonomidis, Ioannis; Mokso, Rajmund; Stampanoni, Marco; Roth-Kleiner, Matthias; Schittny, Johannes C (2017). Automated computer-assisted quantitative analysis of intact murine lungs at the alveolar scale. PLoS ONE, 12(9):e0183979.

Abstract

Using state-of-the-art X-ray tomographic microscopy we can image lung tissue in three dimensions in intact animals down to a micrometer precision. The structural complexity and hierarchical branching scheme of the lung at this level of details, however, renders the extraction of biologically relevant quantities particularly challenging. We have developed a methodology for a detailed description of lung inflation patterns by measuring the size and the local curvature of the parenchymal airspaces. These quantitative tools for morphological and topological analyses were applied to high-resolution murine 3D lung image data, inflated at different pressure levels under immediate post mortem conditions. We show for the first time direct indications of heterogeneous intra-lobar and inter-lobar distension patterns at the alveolar level. Furthermore, we did not find any indication that a cyclic opening-and-collapse (recruitment) of a large number of alveoli takes place.

Abstract

Using state-of-the-art X-ray tomographic microscopy we can image lung tissue in three dimensions in intact animals down to a micrometer precision. The structural complexity and hierarchical branching scheme of the lung at this level of details, however, renders the extraction of biologically relevant quantities particularly challenging. We have developed a methodology for a detailed description of lung inflation patterns by measuring the size and the local curvature of the parenchymal airspaces. These quantitative tools for morphological and topological analyses were applied to high-resolution murine 3D lung image data, inflated at different pressure levels under immediate post mortem conditions. We show for the first time direct indications of heterogeneous intra-lobar and inter-lobar distension patterns at the alveolar level. Furthermore, we did not find any indication that a cyclic opening-and-collapse (recruitment) of a large number of alveoli takes place.

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Item Type:Journal Article["page:refereed_set_refereed" not defined]["page:subtype_original" not defined]
Communities & Collections:04 Faculty of Medicine > Institute of Biomedical Engineering
Dewey Decimal Classification:170 Ethics
610 Medicine & health
Language:English
Date:2017
Deposited On:22 Mar 2018 11:26
Last Modified:13 Apr 2018 11:44
Publisher:Public Library of Science (PLoS)
ISSN:1932-6203
OA Status:Gold
Free access at:PubMed ID. ["page:embargo_may_apply" not defined]
Publisher DOI:https://doi.org/10.1371/journal.pone.0183979
PubMed ID:28934236

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