Header

UZH-Logo

Maintenance Infos

Prion protein expression in different species: analysis with a panel of new mAbs.


Zanusso, G; Liu, D; Ferrari, S; Hegyi, I; Yin, X; Aguzzi, A; Hornemann, S; Liemann, S; Glockshuber, R; Manson, J C; Brown, P; Petersen, R B; Gambetti, P; Sy, M S (1998). Prion protein expression in different species: analysis with a panel of new mAbs. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 95(15):8812-8816.

Abstract

By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrPc), we obtained a panel of mAbs specific for murine PrPc. These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrPc) and protease-resistant (PrPres) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPc. These newly generated PrPc antibodies will help to explore the biology of PrPc and to establish the diagnosis of prion diseases in both humans and animals.

Abstract

By immunizing prion knockout mice (Prnp-/-) with recombinant murine prion protein (PrPc), we obtained a panel of mAbs specific for murine PrPc. These mAbs can be applied to immunoblotting, cell surface immunofluorescent staining, and immunohistochemistry at light and electron microscopy. These mAbs recognize both the normal (PrPc) and protease-resistant (PrPres) isoforms of PrP. Some mAbs are species restricted, while others react with PrP from a broad range of mammals including mice, humans, monkeys, cows, sheep, squirrels, and hamsters. Moreover, some of the mAbs selectively recognize different PrP glycoforms as well as the metabolic fragments of PrPc. These newly generated PrPc antibodies will help to explore the biology of PrPc and to establish the diagnosis of prion diseases in both humans and animals.

Statistics

Citations

166 citations in Web of Science®
164 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

97 downloads since deposited on 11 Feb 2008
33 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Institute of Neuropathology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:21 July 1998
Deposited On:11 Feb 2008 12:25
Last Modified:21 Nov 2017 13:24
Publisher:National Academy of Sciences
ISSN:0027-8424
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1073/pnas.95.15.8812
Related URLs:http://www.pnas.org/cgi/content/abstract/95/15/8812
PubMed ID:9671761

Download

Download PDF  'Prion protein expression in different species: analysis with a panel of new mAbs.'.
Preview
Filetype: PDF
Size: 310kB
View at publisher
Download PDF  'Prion protein expression in different species: analysis with a panel of new mAbs.'.
Preview
Filetype: PDF
Size: 310kB