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Protein dynamics from single-molecule fluorescence intensity correlation functions


Gopich, I V; Nettels, D; Schuler, B; Szabo, A (2009). Protein dynamics from single-molecule fluorescence intensity correlation functions. Journal of Chemical Physics, 131(9):095102.

Abstract

Fluorescence intensity correlation functions contain information about photophysical and conformational dynamics. We propose and implement a simple procedure to analyze such functions measured in the presence of resonance energy transfer. When there is a separation of time scales and the conformational dynamics is modeled as diffusion in the potential of mean force along the interdye distance, we obtain an analytic expression for the conformational correlation time. This can be used to find the diffusion coefficient describing conformational fluctuations given the photon count rate and equilibrium distribution.

Abstract

Fluorescence intensity correlation functions contain information about photophysical and conformational dynamics. We propose and implement a simple procedure to analyze such functions measured in the presence of resonance energy transfer. When there is a separation of time scales and the conformational dynamics is modeled as diffusion in the potential of mean force along the interdye distance, we obtain an analytic expression for the conformational correlation time. This can be used to find the diffusion coefficient describing conformational fluctuations given the photon count rate and equilibrium distribution.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:September 2009
Deposited On:12 Oct 2009 09:43
Last Modified:21 Nov 2017 14:19
Publisher:American Institute of Physics
ISSN:0021-9606
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1063/1.3212597
PubMed ID:19739874

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