Header

UZH-Logo

Maintenance Infos

A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms


Gmür, R; Lüthi-Schaller, H (2007). A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms. Journal of Microbiological Methods, 69(2):402-405.

Abstract

A IF-FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enumeration of bacterial populations with distinct 16S rRNA sequences and/or surface markers of interest.

Abstract

A IF-FISH protocol was developed to characterize discrete cell populations within complex dental plaque samples. Optimizing and shortening the 3-step IF procedure and including RNase inhibitor in all IF reagents enabled the combination of both techniques and thus the simultaneous detection and enumeration of bacterial populations with distinct 16S rRNA sequences and/or surface markers of interest.

Statistics

Citations

16 citations in Web of Science®
15 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

127 downloads since deposited on 16 Jun 2008
13 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Institute of Oral Biology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2007
Deposited On:16 Jun 2008 14:54
Last Modified:05 Apr 2016 12:23
Publisher:Elsevier
ISSN:0167-7012
Publisher DOI:https://doi.org/10.1016/j.mimet.2006.12.012
PubMed ID:17258338

Download

Download PDF  'A combined immunofluorescence and fluorescent in situ hybridization assay for single cell analyses of dental plaque microorganisms'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 1MB
View at publisher