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Identification of Enterobacteriaceae isolates from raw ingredients, environmental samples and products of an infant formula processing plant


Popp, A; Iversen, C; Fricker-Feer, C; Gschwend, G; Stephan, R (2009). Identification of Enterobacteriaceae isolates from raw ingredients, environmental samples and products of an infant formula processing plant. Archiv für Lebensmittelhygiene, 60(3):92-97.

Abstract

Over the past few years the group of Enterobacteriaceae has considerably gained in importance for food industries, especially for infant formula processing plants.
Up to now, only a few studies have focused on the diversity of this group in powdered infant formula and related products. Therefore, the aim of this study was to identify Enterobacteriaceae isolates from a previous study from different samples of an infant formula processing plant. A total of 470 isolates from raw ingredients (n = 117), environmental samples (n = 166) and finished products (n = 187) were further identified by biochemical tests as well as by rpoB sequencing. The most common species was Enterobacter cloacae (n = 161), followed by Pantoea
spp. (n = 51) and Klebsiella pneumoniae (n = 39). In total, 65 isolates could not be identified. Using the partial rpoB gene sequence data as a similarity matrix, 22 different clusters could be formed within these 65 isolates. The fact that some isolates from the same rpoB cluster originate from different sample types might
indicate that they are widely common within such a production environment and hence of practical relevance. These isolates which form possibly novel genus/species will be further investigated.

Abstract

Over the past few years the group of Enterobacteriaceae has considerably gained in importance for food industries, especially for infant formula processing plants.
Up to now, only a few studies have focused on the diversity of this group in powdered infant formula and related products. Therefore, the aim of this study was to identify Enterobacteriaceae isolates from a previous study from different samples of an infant formula processing plant. A total of 470 isolates from raw ingredients (n = 117), environmental samples (n = 166) and finished products (n = 187) were further identified by biochemical tests as well as by rpoB sequencing. The most common species was Enterobacter cloacae (n = 161), followed by Pantoea
spp. (n = 51) and Klebsiella pneumoniae (n = 39). In total, 65 isolates could not be identified. Using the partial rpoB gene sequence data as a similarity matrix, 22 different clusters could be formed within these 65 isolates. The fact that some isolates from the same rpoB cluster originate from different sample types might
indicate that they are widely common within such a production environment and hence of practical relevance. These isolates which form possibly novel genus/species will be further investigated.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2009
Deposited On:11 Jan 2010 16:18
Last Modified:05 Apr 2016 13:38
Publisher:M.& H. Schaper
ISSN:0003-925X
Publisher DOI:https://doi.org/10.2376/0003-925X-60-92

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