Background. Positive psychology studies what is best in people. In their classification of strengths and virtues, Peterson and Seligman  assign humor to the virtue of transcendence. Thus far, there is no specific study that deals with age-related changes in humor (as a strength of character) across a lifespan and its relation to well-being in the elderly. Participants and methods. A total of n=42,964 participants completed an online questionnaire on humor as a strength of character. Participants also completed the Satisfaction with Life Scale and the Orientations to Happiness Scale.Results. In a cross-sectional design, the scores for humor decreased until the age of 50. Men between 51 and 62 years had higher scores; there was a trend for women older than 70 to score higher (but this was not statistically significant). Humor was robustly positively correlated with life satisfaction, as well with a pleasurable and an engaged life, but was lowest with a meaningful life. The oldest participants had the lowest (yet still meaningful) correlation coefficients (with the exception of a meaningful life).Conclusion. The study contributes to the understanding of humor across the lifespan and underlines the importance of studies among the elderly within a framework of positive psychology.