Header

UZH-Logo

Maintenance Infos

Assessment of six different collagenase-based methods to isolate feline pancreatic islets


Zini, E; Franchini, M; Guscetti, F; Osto, M; Kaufmann, K; Ackermann, M; Lutz, T A; Reusch, C E (2009). Assessment of six different collagenase-based methods to isolate feline pancreatic islets. Research in Veterinary Science, 87(3):367-372.

Abstract

Isolation of pancreatic islets is necessary to study the molecular mechanisms underlying beta-cell demise in diabetic cats. Six collagenase-based methods of isolation were compared in 10 cat pancreata, including single and double course of collagenase, followed or not by Ficoll centrifugation or accutase, and collagenase plus accutase. Morphometric analysis was performed to measure the relative area of islet and exocrine tissue. Islet specific mRNA transcripts were quantified in isolates by real-time PCR. The single and double course of collagenase digestion was successful in each cat and provided similar islet-to-exocrine tissue ratio. Quantities of insulin mRNA did not differ between the two methods. However, on histological examination either method yielded only approximately 2% of pure islets. The other methods provided disrupted islets or insufficient samples in 1-7 cats. Although pancreas digestion with single and double course of collagenase was superior, further studies are needed to improve islet isolation in cats.

Abstract

Isolation of pancreatic islets is necessary to study the molecular mechanisms underlying beta-cell demise in diabetic cats. Six collagenase-based methods of isolation were compared in 10 cat pancreata, including single and double course of collagenase, followed or not by Ficoll centrifugation or accutase, and collagenase plus accutase. Morphometric analysis was performed to measure the relative area of islet and exocrine tissue. Islet specific mRNA transcripts were quantified in isolates by real-time PCR. The single and double course of collagenase digestion was successful in each cat and provided similar islet-to-exocrine tissue ratio. Quantities of insulin mRNA did not differ between the two methods. However, on histological examination either method yielded only approximately 2% of pure islets. The other methods provided disrupted islets or insufficient samples in 1-7 cats. Although pancreas digestion with single and double course of collagenase was superior, further studies are needed to improve islet isolation in cats.

Statistics

Citations

1 citation in Web of Science®
3 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

316 downloads since deposited on 18 Jan 2010
26 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Physiology
05 Vetsuisse Faculty > Institute of Veterinary Pathology
05 Vetsuisse Faculty > Institute of Virology
05 Vetsuisse Faculty > Veterinary Clinic > Department of Small Animals
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2009
Deposited On:18 Jan 2010 15:11
Last Modified:21 Nov 2017 14:33
Publisher:Elsevier
ISSN:0034-5288
Publisher DOI:https://doi.org/10.1016/j.rvsc.2009.03.017
PubMed ID:19386336

Download

Download PDF  'Assessment of six different collagenase-based methods to isolate feline pancreatic islets'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 1MB
View at publisher