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Nanoscale topography-induced modulation of fundamental cell behaviors of rabbit corneal keratocytes, fibroblasts, and myofibroblasts


Pot, S A; Liliensiek, S J; Myrna, K E; Bentley, E; Jester, J V; Nealey, P F; Murphy, C J (2010). Nanoscale topography-induced modulation of fundamental cell behaviors of rabbit corneal keratocytes, fibroblasts, and myofibroblasts. Investigative Ophthalmology and Visual Science, 51(3):1373-1381.

Abstract

Purpose: Keratocyte to myofibroblast differentiation is a key factor in corneal wound healing. The purpose of this study was to determine the influence of environmental nanoscale topography on keratocyte, fibroblast and myofibroblast cell behavior. Methods: Primary rabbit corneal keratocytes, fibroblasts and myofibroblasts were seeded onto planar polyurethane surfaces with six patterned
areas, composed of anisotropically ordered grooves and ridges with a 400, 800, 1200, 1600, 2000 and 4000 nm pitch (pitch = groove + ridge width). After 24 hours cells were fixed, stained, imaged and analyzed for cell shape and
orientation. For migration studies, cells on each patterned surface were imaged every 10 minutes for 12 hours and individual cell trajectories and migration rates were calculated. Results: Keratocytes, fibroblasts and myofibroblasts aligned and elongated to pitch sizes > 1000 nm. A lower limit to the topographic feature sizes that the cells responded to was identified for all three phenotypes with a transition zone around the 800-1200 nm pitch size. Fibroblasts and myofibroblasts migrated parallel to surface ridges > 1000 nm, but lacked directional guidance on submicron and nanoscale topographic features as well
as on planar surfaces. Keratocytes remained essentially immobile. Conclusions: Corneal stromal cells elongated, aligned and migrated differentially guided by substratum topographic features. All cell types failed to respond to individual stromal fibers. These findings contribute to our understanding of corneal stromal cell biology in health and disease and their interaction with biomaterials and their native extracellular matrix.

Abstract

Purpose: Keratocyte to myofibroblast differentiation is a key factor in corneal wound healing. The purpose of this study was to determine the influence of environmental nanoscale topography on keratocyte, fibroblast and myofibroblast cell behavior. Methods: Primary rabbit corneal keratocytes, fibroblasts and myofibroblasts were seeded onto planar polyurethane surfaces with six patterned
areas, composed of anisotropically ordered grooves and ridges with a 400, 800, 1200, 1600, 2000 and 4000 nm pitch (pitch = groove + ridge width). After 24 hours cells were fixed, stained, imaged and analyzed for cell shape and
orientation. For migration studies, cells on each patterned surface were imaged every 10 minutes for 12 hours and individual cell trajectories and migration rates were calculated. Results: Keratocytes, fibroblasts and myofibroblasts aligned and elongated to pitch sizes > 1000 nm. A lower limit to the topographic feature sizes that the cells responded to was identified for all three phenotypes with a transition zone around the 800-1200 nm pitch size. Fibroblasts and myofibroblasts migrated parallel to surface ridges > 1000 nm, but lacked directional guidance on submicron and nanoscale topographic features as well
as on planar surfaces. Keratocytes remained essentially immobile. Conclusions: Corneal stromal cells elongated, aligned and migrated differentially guided by substratum topographic features. All cell types failed to respond to individual stromal fibers. These findings contribute to our understanding of corneal stromal cell biology in health and disease and their interaction with biomaterials and their native extracellular matrix.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Equine Department
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Language:English
Date:March 2010
Deposited On:22 Jan 2010 14:59
Last Modified:03 Aug 2017 15:12
Publisher:Association for Research in Vision and Ophthalmology
ISSN:0146-0404
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1167/iovs.09-4074
PubMed ID:19875665

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