Header

UZH-Logo

Maintenance Infos

Selenium functionalized intraocular lenses inhibit posterior capsule opacification in an ex vivo canine lens capsular bag assay


Pot, Simon A; Chandler, H L; Colitz, C M H; Bentley, E; Dubielzig, R R; Mosley, T S; Reid, T W; Murphy, C J (2009). Selenium functionalized intraocular lenses inhibit posterior capsule opacification in an ex vivo canine lens capsular bag assay. Experimental Eye Research, 89(5):728-734.

Abstract

The purpose of this study was to determine the inhibitory effect of selenocystamine coated intraocularlenses (IOLs) on the formation of posterior capsule opacification (PCO) in an ex vivo canine lens capsularbag assay.Selenocystamine was covalently bound to the surface of poly(2-hydroxyethyl methacrylate)(poly(HEMA)) discs. Three groups of canine lens capsules (6 coated IOLs (SeIOLs), 7 non-coated control
IOLs and 8 empty capsules) were cultured for 10 days. During the culture period PCO was scored basedon visual inspection of the capsules using phase contrast microscopy. On day 10 all the capsules were prepared for light microscopic examination and lens epithelial cells (LECs) were quantified. Proliferating cell nuclear antigen (PCNA), a-smooth muscle actin (a-SMA) and cleaved caspase-3 were examined by immunohistochemistry. Additionally, cell viability assays were performed on LECs cultured in tissue
culture medium pre-incubated with either a SeIOL or control IOL. The viability assays demonstrated that no detectable cytotoxic leachables were associated with the functionalized IOLs. The central posterior capsule was free of cells underneath all SeIOLs, although large numbers of LECs populated the capsular periphery. Apoptotic cells were observed underneath the periphery of some SeIOLs. Both the PCO scores and LEC counts of SeIOL containing capsules were significantly lower than those of control group capsules (p < 0.01 and p ¼ 0.0004, respectively).
The use of selenium functionalized IOLs resulted in a significant reduction of PCO in this ex vivo model.
Binding of selenocystamine to a foldable IOL may provide an effective method to prevent population of the central posterior capsule with LECs.

Abstract

The purpose of this study was to determine the inhibitory effect of selenocystamine coated intraocularlenses (IOLs) on the formation of posterior capsule opacification (PCO) in an ex vivo canine lens capsularbag assay.Selenocystamine was covalently bound to the surface of poly(2-hydroxyethyl methacrylate)(poly(HEMA)) discs. Three groups of canine lens capsules (6 coated IOLs (SeIOLs), 7 non-coated control
IOLs and 8 empty capsules) were cultured for 10 days. During the culture period PCO was scored basedon visual inspection of the capsules using phase contrast microscopy. On day 10 all the capsules were prepared for light microscopic examination and lens epithelial cells (LECs) were quantified. Proliferating cell nuclear antigen (PCNA), a-smooth muscle actin (a-SMA) and cleaved caspase-3 were examined by immunohistochemistry. Additionally, cell viability assays were performed on LECs cultured in tissue
culture medium pre-incubated with either a SeIOL or control IOL. The viability assays demonstrated that no detectable cytotoxic leachables were associated with the functionalized IOLs. The central posterior capsule was free of cells underneath all SeIOLs, although large numbers of LECs populated the capsular periphery. Apoptotic cells were observed underneath the periphery of some SeIOLs. Both the PCO scores and LEC counts of SeIOL containing capsules were significantly lower than those of control group capsules (p < 0.01 and p ¼ 0.0004, respectively).
The use of selenium functionalized IOLs resulted in a significant reduction of PCO in this ex vivo model.
Binding of selenocystamine to a foldable IOL may provide an effective method to prevent population of the central posterior capsule with LECs.

Statistics

Citations

8 citations in Web of Science®
7 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

1 download since deposited on 18 Jan 2010
0 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Equine Department
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Language:English
Date:2009
Deposited On:18 Jan 2010 14:53
Last Modified:06 Dec 2017 23:25
Publisher:Academic Press
ISSN:0014-4835
Publisher DOI:https://doi.org/10.1016/j.exer.2009.06.016
PubMed ID:19583956

Download