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Deep UV sensing of the interaction of porphyrin with bovine serum albumin protein


Li, Q; Seeger, S (2009). Deep UV sensing of the interaction of porphyrin with bovine serum albumin protein. Sensors and Actuators B: Chemical, 139(1):118-124.

Abstract

The interaction of meso-tetrakis(p-sulfonatophenyl)porphyrin (TPPS) with bovine serum albumin (BSA) in neutral solution (pH 7.4) has been studied by means of absorption, steady-state fluorescence and time-resolved fluorescence spectroscopy. The formation of TPPS–BSA complex was monitored by spectroscopic characteristic changes in Soret band absorption and fluorescence emission of TPPS. Applying the time-correlated single-photon counting (TCSPC) method, BSA–TPPS interaction was also investigated by the fluorescence lifetime of tryptophan residues in BSA. The results demonstrate that deep UV laser-based fluorescence lifetime microscopy is useful for sensitive identification of protein interaction using intrinsic fluorescence.

Abstract

The interaction of meso-tetrakis(p-sulfonatophenyl)porphyrin (TPPS) with bovine serum albumin (BSA) in neutral solution (pH 7.4) has been studied by means of absorption, steady-state fluorescence and time-resolved fluorescence spectroscopy. The formation of TPPS–BSA complex was monitored by spectroscopic characteristic changes in Soret band absorption and fluorescence emission of TPPS. Applying the time-correlated single-photon counting (TCSPC) method, BSA–TPPS interaction was also investigated by the fluorescence lifetime of tryptophan residues in BSA. The results demonstrate that deep UV laser-based fluorescence lifetime microscopy is useful for sensitive identification of protein interaction using intrinsic fluorescence.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Chemistry
Dewey Decimal Classification:540 Chemistry
Language:English
Date:2009
Deposited On:30 Jan 2010 12:18
Last Modified:26 Jan 2017 08:46
Publisher:Elsevier
ISSN:0925-4005
Publisher DOI:https://doi.org/10.1016/j.snb.2008.10.001

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