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Development of polymorphic microsatellite markers for the livebearing fish 'Poecilia parae'


Nater, A; Krützen, M; Lindholm, A K (2008). Development of polymorphic microsatellite markers for the livebearing fish 'Poecilia parae'. Molecular Ecology Resources, 8(4):857-860.

Abstract

We developed 16 novel polymorphic tetranucleotide microsatellite markers for Poecilia parae, a livebearing fish used in evolutionary studies because of its Y-linked colour and size polymorphism. A set of 199 clones was sequenced out of an enriched genomic library, and we achieved an enrichment efficiency of nearly 80%. Primers were designed for 16 pure repeats, and 59 P. parae were screened for polymorphism. Cross-amplification was tested on Poecilia picta and Poecilia reticulata, the guppy. The new microsatellite markers showed an exceptionally high allelic diversity and low stutter formation, proving their suitability for a broad range of applications in these species.

Abstract

We developed 16 novel polymorphic tetranucleotide microsatellite markers for Poecilia parae, a livebearing fish used in evolutionary studies because of its Y-linked colour and size polymorphism. A set of 199 clones was sequenced out of an enriched genomic library, and we achieved an enrichment efficiency of nearly 80%. Primers were designed for 16 pure repeats, and 59 P. parae were screened for polymorphism. Cross-amplification was tested on Poecilia picta and Poecilia reticulata, the guppy. The new microsatellite markers showed an exceptionally high allelic diversity and low stutter formation, proving their suitability for a broad range of applications in these species.

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6 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
07 Faculty of Science > Department of Anthropology
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
300 Social sciences, sociology & anthropology
Uncontrolled Keywords:Poecilia parae, microsatellites, genetic marker, paternity testing
Language:English
Date:2008
Deposited On:04 Aug 2008 09:58
Last Modified:05 Apr 2016 12:25
Publisher:Wiley-Blackwell
ISSN:1755-098X
Publisher DOI:https://doi.org/10.1111/j.1755-0998.2008.02090.x

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