Header

UZH-Logo

Maintenance Infos

Selection of antibodies for immunohistochemical detection of Bcl-2 family members in dogs and immunohistochemical expression pattern of Mcl-1 and Bcl-x in canine normal organs and lymphomas


Zeller, S. Selection of antibodies for immunohistochemical detection of Bcl-2 family members in dogs and immunohistochemical expression pattern of Mcl-1 and Bcl-x in canine normal organs and lymphomas. 2009, University of Zurich, Vetsuisse Faculty.

Abstract

The Bcl-2 family plays an important role in tumourigenesis but its role in the genesis of canine lymphoma is yet unexplored. The aims of the present study were to select antibodies for immunohistochemical detection of various Bcl-2 family members in canine tissues and to analyse the expression of two anti-apoptotic members, Mcl-1 and Bcl-x, in normal canine and lymphoma tissues. A total of twelve antibodies out of a panel of twenty-one were deemed to specifically cross-react with canine recombinant Mcl-1, Bcl-x, Bcl-w, Bak and Bax expressed in bacteria. This was determined using an immunohistochemical method in formalin-fixed, paraffin-embedded bacteria and confirmatory Western blotting. Immunohistochemical protocols for Mcl-1 and Bcl-x were further optimized for use in canine tissues using cultured canine cells and selected normal tissues fixed in formalin and embedded in paraffin. Immunohistochemical expression patterns of these two proteins in normal canine tissues, detected using tissue arrays, are described here for the first time. They roughly coincide with the expression patterns reported in human tissues. With the same techniques, canine lymphomas were shown to frequently express Mcl-1 and, to a lesser extent, Bcl-x, across a wide range of different subtypes. These findings suggest a role for these two anti-apoptotic Bcl-2 family members in the pathogenesis of canine lymphoma.

Abstract

The Bcl-2 family plays an important role in tumourigenesis but its role in the genesis of canine lymphoma is yet unexplored. The aims of the present study were to select antibodies for immunohistochemical detection of various Bcl-2 family members in canine tissues and to analyse the expression of two anti-apoptotic members, Mcl-1 and Bcl-x, in normal canine and lymphoma tissues. A total of twelve antibodies out of a panel of twenty-one were deemed to specifically cross-react with canine recombinant Mcl-1, Bcl-x, Bcl-w, Bak and Bax expressed in bacteria. This was determined using an immunohistochemical method in formalin-fixed, paraffin-embedded bacteria and confirmatory Western blotting. Immunohistochemical protocols for Mcl-1 and Bcl-x were further optimized for use in canine tissues using cultured canine cells and selected normal tissues fixed in formalin and embedded in paraffin. Immunohistochemical expression patterns of these two proteins in normal canine tissues, detected using tissue arrays, are described here for the first time. They roughly coincide with the expression patterns reported in human tissues. With the same techniques, canine lymphomas were shown to frequently express Mcl-1 and, to a lesser extent, Bcl-x, across a wide range of different subtypes. These findings suggest a role for these two anti-apoptotic Bcl-2 family members in the pathogenesis of canine lymphoma.

Statistics

Downloads

196 downloads since deposited on 10 Feb 2010
13 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Dissertation
Referees:Guscetti F, Boos A
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Pathology
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2009
Deposited On:10 Feb 2010 16:08
Last Modified:07 Dec 2017 00:15
Number of Pages:69
Additional Information:Selection of antibodies for immunohistochemical detection of Bcl-2 family members in dogs and immunohistochemical expression pattern of Mcl-1 and Bcl-x in canine normal organs and lymphomas / vorgelegt von Sarah Zeller. - Zürich, 2009
Related URLs:http://opac.nebis.ch/F/?local_base=NEBIS&con_lng=GER&func=find-b&find_code=SYS&request=005948494

Download

Download PDF  'Selection of antibodies for immunohistochemical detection of Bcl-2 family members in dogs and immunohistochemical expression pattern of Mcl-1 and Bcl-x in canine normal organs and lymphomas'.
Preview
Filetype: PDF
Size: 85MB