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Evaluation of the Sysmex XT-2000iV hematology instrument for use with feline blood


Weissenbacher, S. Evaluation of the Sysmex XT-2000iV hematology instrument for use with feline blood. 2009, University of Zurich, Vetsuisse Faculty.

Abstract

The objective of this study was to evaluate the Sysmex XT-2000iV with respect to its use with samples from the feline species.
The Sysmex XT-2000iV is a flow cytometer that analyzes 31 hematological parameters and is based on the principles of multi-angle polarized light scatter separation for WBC, WBC differential, RET and PLT-O. In addition, the impedance method is used to determine the parameters RBC and PLT-I, and SLS (sodium lauryl sulfate) is used for HGB.
The reference methods used were those recommended by the International Committee for Standardization in Haematology (ICSH). These included centrifugal hematocrit, the CellDyn® 3500 analyzer for RBC, red cell indices, and WBC, microscopic differentiation of 200 WBC (100 cells each by two technicians), and manual RET examination.
The coefficients of variation for precision in series were good to excellent and for precision from day to day excellent.
The tested range of linearity extended the reference ranges up to 100x103/μl for WBC, 19.6x106/μl for RBC, 29.1 g/dl for HGB, 82% for HCT, 98.2x103/μl for RET, and 4140x103/μl for PLT.
The correlations between the Sysmex XT-2000iV and the reference methods were good to excellent, except for MONO# (r = 0.65).
Additionally, quantitation of RET allowed characterization of regenerative versus nonregenerative anemias.
From this evaluation, we conclude that the Sysmex XT-2000iV is an excellent and highly reliable hematology instrument for the analysis of feline blood samples.

Abstract

The objective of this study was to evaluate the Sysmex XT-2000iV with respect to its use with samples from the feline species.
The Sysmex XT-2000iV is a flow cytometer that analyzes 31 hematological parameters and is based on the principles of multi-angle polarized light scatter separation for WBC, WBC differential, RET and PLT-O. In addition, the impedance method is used to determine the parameters RBC and PLT-I, and SLS (sodium lauryl sulfate) is used for HGB.
The reference methods used were those recommended by the International Committee for Standardization in Haematology (ICSH). These included centrifugal hematocrit, the CellDyn® 3500 analyzer for RBC, red cell indices, and WBC, microscopic differentiation of 200 WBC (100 cells each by two technicians), and manual RET examination.
The coefficients of variation for precision in series were good to excellent and for precision from day to day excellent.
The tested range of linearity extended the reference ranges up to 100x103/μl for WBC, 19.6x106/μl for RBC, 29.1 g/dl for HGB, 82% for HCT, 98.2x103/μl for RET, and 4140x103/μl for PLT.
The correlations between the Sysmex XT-2000iV and the reference methods were good to excellent, except for MONO# (r = 0.65).
Additionally, quantitation of RET allowed characterization of regenerative versus nonregenerative anemias.
From this evaluation, we conclude that the Sysmex XT-2000iV is an excellent and highly reliable hematology instrument for the analysis of feline blood samples.

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Additional indexing

Item Type:Dissertation
Referees:Lutz H, Lutz T
Communities & Collections:05 Vetsuisse Faculty > Veterinary Clinic > Department of Farm Animals
Dewey Decimal Classification:570 Life sciences; biology
630 Agriculture
Date:2009
Deposited On:24 Feb 2010 14:27
Last Modified:14 Sep 2016 13:42
Number of Pages:22

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