Header

UZH-Logo

Maintenance Infos

Lack of mismatch correction facilitates genome evolution in mycobacteria


Springer, B; Sander, P; Sedlacek, L; Hardt, W D; Mizrahi, V; Schärer, P; Böttger, E C (2004). Lack of mismatch correction facilitates genome evolution in mycobacteria. Molecular Microbiology, 53(6):1601-1609.

Abstract

In silico genome sequence analyses suggested that mycobacteria are devoid of the highly conserved mutLS-based post-replicative mismatch repair system. Here, we present the first biological evidence for the lack of a classical mismatch repair function in mycobacteria. We found that frameshifts, but not general mutation rates are unusually high in Mycobacterium smegmatis. However, despite the absence of mismatch correction, M. smegmatis establishes a strong barrier to recombination between homeologous DNA sequences. We show that 10-12% of DNA sequence heterology restricts initiation of recombination but not extension of heteroduplex DNA intermediates. Together, the lack of mismatch correction and a high stringency of initiation of homologous recombination provide an adequate strategy for mycobacterial genome evolution, which occurs by gene duplication and divergent evolution.

Abstract

In silico genome sequence analyses suggested that mycobacteria are devoid of the highly conserved mutLS-based post-replicative mismatch repair system. Here, we present the first biological evidence for the lack of a classical mismatch repair function in mycobacteria. We found that frameshifts, but not general mutation rates are unusually high in Mycobacterium smegmatis. However, despite the absence of mismatch correction, M. smegmatis establishes a strong barrier to recombination between homeologous DNA sequences. We show that 10-12% of DNA sequence heterology restricts initiation of recombination but not extension of heteroduplex DNA intermediates. Together, the lack of mismatch correction and a high stringency of initiation of homologous recombination provide an adequate strategy for mycobacterial genome evolution, which occurs by gene duplication and divergent evolution.

Statistics

Citations

53 citations in Web of Science®
60 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

3 downloads since deposited on 22 Aug 2008
0 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Medical Microbiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Date:August 2004
Deposited On:22 Aug 2008 12:31
Last Modified:05 Apr 2016 12:26
Publisher:Wiley-Blackwell
ISSN:0950-382X
Publisher DOI:https://doi.org/10.1111/j.1365-2958.2004.04231.x
PubMed ID:15341642

Download