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Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples


Urban, P L; Amantonico, A; Fagerer, S R; Gehrig, P; Zenobi, R (2010). Mass spectrometric method incorporating enzymatic amplification for attomole-level analysis of target metabolites in biological samples. Chemical Communications, 46(13):2212-2214.

Abstract

We report a sensitive method for selective detection of target metabolites from the central metabolic pathway by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), in which the MS signal is enhanced by up to three orders of magnitude in the course of in situ enzymatic amplification.

Abstract

We report a sensitive method for selective detection of target metabolites from the central metabolic pathway by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS), in which the MS signal is enhanced by up to three orders of magnitude in the course of in situ enzymatic amplification.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Functional Genomics Center Zurich
08 University Research Priority Programs > Systems Biology / Functional Genomics
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2010
Deposited On:04 Nov 2010 16:04
Last Modified:17 Feb 2018 17:20
Publisher:Royal Society of Chemistry
ISSN:1359-7345
OA Status:Closed
Free access at:Related URL. An embargo period may apply.
Publisher DOI:https://doi.org/10.1039/b925433a
Related URLs:http://www.zenobi.ethz.ch/publications/2010/2010%20Chem%20Comm-Urban.pdf
PubMed ID:20234909

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