Header

UZH-Logo

Maintenance Infos

Evolution of repeated sequence arrays in the D-loop region of bat mitochondrial DNA.


Wilkinson, G S; Mayer, F; Kerth, G; Petri, B (1997). Evolution of repeated sequence arrays in the D-loop region of bat mitochondrial DNA. Genetics, 146(3):1035-1048.

Abstract

Analysis of mitochondrial DNA control region sequences from 41 species of bats representing 11 families revealed that repeated sequence arrays near the tRNA-Pro gene are present in all vespertilionine bats. Across 18 species tandem repeats varied in size from 78 to 85 bp and contained two to nine repeats. Heteroplasmy ranged from 15% to 63%. Fewer repeats among heteroplasmic than homoplasmic individuals in a species with up to nine repeats indicates selection may act against long arrays. A lower limit of two repeats and more repeats among heteroplasmic than homoplasmic individuals in two species with few repeats suggests length mutations are biased. Significant regressions of heteroplasmy, theta and pi, on repeat number further suggest that repeat duplication rate increases with repeat number. Comparison of vespertilionine bat consensus repeats to mammal control region sequences revealed that tandem repeats of similar size, sequence and number also occur in shrews, cats and bighorn sheep. The presence of two conserved protein-binding sequences in all repeat units indicates that convergent evolution has occurred by duplication of functional units. We speculate that D-loop region tandem repeats may provide signal redundancy and a primitive repair mechanism in the event of somatic mutations to these binding sites.

Abstract

Analysis of mitochondrial DNA control region sequences from 41 species of bats representing 11 families revealed that repeated sequence arrays near the tRNA-Pro gene are present in all vespertilionine bats. Across 18 species tandem repeats varied in size from 78 to 85 bp and contained two to nine repeats. Heteroplasmy ranged from 15% to 63%. Fewer repeats among heteroplasmic than homoplasmic individuals in a species with up to nine repeats indicates selection may act against long arrays. A lower limit of two repeats and more repeats among heteroplasmic than homoplasmic individuals in two species with few repeats suggests length mutations are biased. Significant regressions of heteroplasmy, theta and pi, on repeat number further suggest that repeat duplication rate increases with repeat number. Comparison of vespertilionine bat consensus repeats to mammal control region sequences revealed that tandem repeats of similar size, sequence and number also occur in shrews, cats and bighorn sheep. The presence of two conserved protein-binding sequences in all repeat units indicates that convergent evolution has occurred by duplication of functional units. We speculate that D-loop region tandem repeats may provide signal redundancy and a primitive repair mechanism in the event of somatic mutations to these binding sites.

Statistics

Citations

99 citations in Web of Science®
99 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

106 downloads since deposited on 11 Feb 2008
9 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed
Communities & Collections:07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
Language:English
Date:1997
Deposited On:11 Feb 2008 12:15
Last Modified:03 Aug 2017 14:43
Publisher:Genetics Society of America
ISSN:0016-6731
Free access at:PubMed ID. An embargo period may apply.
Related URLs:http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=9215906
PubMed ID:9215906

Download

Download PDF  'Evolution of repeated sequence arrays in the D-loop region of bat mitochondrial DNA.'.
Preview
Filetype: PDF
Size: 4MB