Header

UZH-Logo

Maintenance Infos

Computer-based analysis of the optokinetic response in zebrafish larvae


Hodel, C; Neuhauss, S C F (2008). Computer-based analysis of the optokinetic response in zebrafish larvae. Cold Spring Harbor Protocols:4961.

Abstract

Large movements in the visual field trigger stereotypic eye movements in vertebrates. This optokinetic response
(OKR) is robust in zebrafish (Danio rerio) larvae older than 4 days post-fertilization (dpf), and is hence ideally
suited for use in the evaluation of visual performance after genetic manipulations. This protocol describes a simple
method by which a computer-generated visual stimulus is projected onto a screen and watched by immobilized
larvae. The resulting eye movements are recorded and evaluated automatically, using a digital video camera and
appropriate computer software. Depending on the sophistication of the software used, the evaluation can be
performed in real time during the recording session, or by analyzing the recorded movie files off-line.

Abstract

Large movements in the visual field trigger stereotypic eye movements in vertebrates. This optokinetic response
(OKR) is robust in zebrafish (Danio rerio) larvae older than 4 days post-fertilization (dpf), and is hence ideally
suited for use in the evaluation of visual performance after genetic manipulations. This protocol describes a simple
method by which a computer-generated visual stimulus is projected onto a screen and watched by immobilized
larvae. The resulting eye movements are recorded and evaluated automatically, using a digital video camera and
appropriate computer software. Depending on the sophistication of the software used, the evaluation can be
performed in real time during the recording session, or by analyzing the recorded movie files off-line.

Statistics

Citations

Altmetrics

Downloads

82 downloads since deposited on 20 Jan 2009
13 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:zebrafish, optokinetic response, visual behavior
Language:English
Date:2008
Deposited On:20 Jan 2009 12:00
Last Modified:06 Dec 2017 14:33
Publisher:Cold Spring Harbor Press
ISSN:1559-6095
Publisher DOI:https://doi.org/10.1101/pdb.prot4961
Official URL:http://cshprotocols.cshlp.org/cgi/content/full/2008/4/pdb.prot4961

Download

Download PDF  'Computer-based analysis of the optokinetic response in zebrafish larvae'.
Preview
Filetype: PDF
Size: 1MB
View at publisher