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Miniantibodies - Zurich Open Repository and Archive


Schaefer, J V; Lindner, P; Plückthun, A (2010). Miniantibodies. In: Kontermann, R; Dübel, S. Antibody Engineering. Berlin Heidelberg, Germany: Springern-Verlag, 85-99.

Abstract

The usual motivation to create a multivalent molecule is to increase its functional affinity (avidity) to a corresponding multimeric antigen structure, which can be a
cell surface, a virus surface or a fibrous polymer. Obviously, no increase in affinity to a soluble monomeric antigen can be expected. However, an increased functional
affinity to a formally monovalent antigen will usually be observed when it is immobilized on a densely packed surface (e.g. on an ELISA plate or a BIAcore
chip). The increased size of the antibody fragment upon multimerization, in conjunction with higher functional affinity, can also lead to improved tumor localization
(Hu et al. 1996; Todorovska et al. 2001; Deyev et al. 2003; Kubetzko et al. 2006). Besides causing an avidity gain, bivalent binding might also result in
agonistic activity, which may or may not be desired in a particular application.

Abstract

The usual motivation to create a multivalent molecule is to increase its functional affinity (avidity) to a corresponding multimeric antigen structure, which can be a
cell surface, a virus surface or a fibrous polymer. Obviously, no increase in affinity to a soluble monomeric antigen can be expected. However, an increased functional
affinity to a formally monovalent antigen will usually be observed when it is immobilized on a densely packed surface (e.g. on an ELISA plate or a BIAcore
chip). The increased size of the antibody fragment upon multimerization, in conjunction with higher functional affinity, can also lead to improved tumor localization
(Hu et al. 1996; Todorovska et al. 2001; Deyev et al. 2003; Kubetzko et al. 2006). Besides causing an avidity gain, bivalent binding might also result in
agonistic activity, which may or may not be desired in a particular application.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2010
Deposited On:26 Jan 2011 11:48
Last Modified:05 Apr 2016 14:38
Publisher:Springern-Verlag
Number:2
ISBN:978-3-642-01146-7
Publisher DOI:https://doi.org/10.1007/978-3-642-01147-4_7
Official URL:http://www.springer.com/new+%26+forthcoming+titles+(default)/book/978-3-642-01146-7

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