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Vasoactive intestinal peptide (VIP)- mediated expression and function of steroidogenic acute regulatory protein (StAR) in granulosa cells


Kowalewski, M P; Dyson, M T; Boos, A; Stocco, D M (2010). Vasoactive intestinal peptide (VIP)- mediated expression and function of steroidogenic acute regulatory protein (StAR) in granulosa cells. Molecular and Cellular Endocrinology, 328(1-2):93-103.

Abstract

VIP is a peptide hormone capable of activating the cAMP/PKA pathway and modifying gonadal steroidogenic capacity. Less is known about the molecular mechanisms of VIP-mediated steroidogenesis and its role in regulating the steroidogenic acute regulatory protein (STAR). We examined the impact of VIP on STAR expression and function in immortalized (KK1) and primary mouse granulosa cells, where VIP strongly upregulated STAR expression and steroidogenesis. Inhibitors of the PKA and PKC pathways suggested that both are activated by VIP. VIP did not efficiently phosphorylate STAR (P-STAR); however, VIP together with cAMP-analogs that activate Type II PKA increased P-STAR and further increased steroidogenesis. Our results suggest that VIP-induced STAR expression and function in granulosa cells result from the preferential activation of Type I PKA. Furthermore, the PKA and PKC pathways appear to converge at regulating VIP-mediated Star transcription and translation.

Abstract

VIP is a peptide hormone capable of activating the cAMP/PKA pathway and modifying gonadal steroidogenic capacity. Less is known about the molecular mechanisms of VIP-mediated steroidogenesis and its role in regulating the steroidogenic acute regulatory protein (STAR). We examined the impact of VIP on STAR expression and function in immortalized (KK1) and primary mouse granulosa cells, where VIP strongly upregulated STAR expression and steroidogenesis. Inhibitors of the PKA and PKC pathways suggested that both are activated by VIP. VIP did not efficiently phosphorylate STAR (P-STAR); however, VIP together with cAMP-analogs that activate Type II PKA increased P-STAR and further increased steroidogenesis. Our results suggest that VIP-induced STAR expression and function in granulosa cells result from the preferential activation of Type I PKA. Furthermore, the PKA and PKC pathways appear to converge at regulating VIP-mediated Star transcription and translation.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Veterinary Anatomy
Dewey Decimal Classification:570 Life sciences; biology
Uncontrolled Keywords:VIP Steroidogenesis STAR Granulosa cells
Language:English
Date:16 July 2010
Deposited On:07 Feb 2011 13:07
Last Modified:05 Apr 2016 14:41
Publisher:Elsevier
ISSN:0303-7207
Publisher DOI:https://doi.org/10.1016/j.mce.2010.07.018
Related URLs:http://www.elsevier.com/locate/mce

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