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PMT (Photomultiplier) Clinostat


Horn, A; Ullrich, O; Huber, K; Hemmersbach, R (2011). PMT (Photomultiplier) Clinostat. Microgravity Science and Technology, 23(1):67-71.

Abstract

In order to enable kinetic online measurements in cell cultures during exposure to altered gravitational stimulation a new device has been constructed. The analysis principle is based on photon counting with a photomultiplier tube (PMT). The system can work in a rotating mode (clinostat principle) as well as in 1 g conditions. Biological verification was successfully performed with a Luminol-based luminescence measurement of the immune reaction of mammal macrophages. An identical methodical approach is foreseen for an ISS experiment (TRIPLE LUX). Alteration of the rotation speed of the PMT clinostat (60 and 2 rotations per minute—rpm) resulted in a speed-dependent decrease of the luminescent signal, contributing to the current discussion whether and how fast rotation of a sample around one axis perpendicular to the direction of the g-vector provides the condition of functional weightlessness or omnilateral mechano-(gravi-) stimulation.

Abstract

In order to enable kinetic online measurements in cell cultures during exposure to altered gravitational stimulation a new device has been constructed. The analysis principle is based on photon counting with a photomultiplier tube (PMT). The system can work in a rotating mode (clinostat principle) as well as in 1 g conditions. Biological verification was successfully performed with a Luminol-based luminescence measurement of the immune reaction of mammal macrophages. An identical methodical approach is foreseen for an ISS experiment (TRIPLE LUX). Alteration of the rotation speed of the PMT clinostat (60 and 2 rotations per minute—rpm) resulted in a speed-dependent decrease of the luminescent signal, contributing to the current discussion whether and how fast rotation of a sample around one axis perpendicular to the direction of the g-vector provides the condition of functional weightlessness or omnilateral mechano-(gravi-) stimulation.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Anatomy
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2011
Deposited On:17 Feb 2011 08:23
Last Modified:05 Apr 2016 14:46
Publisher:Springer
ISSN:0938-0108
Publisher DOI:https://doi.org/10.1007/s12217-010-9234-5

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