Header

UZH-Logo

Maintenance Infos

Elucidating the role of matrix stiffness in 3D cell migration and remodeling


Ehrbar, M; Sala, A; Lienemann, P; Ranga, A; Mosiewicz, K; Bittermann, A; Rizzi, S C; Weber, Franz E; Lutolf, M P (2011). Elucidating the role of matrix stiffness in 3D cell migration and remodeling. Biophysical Journal, 100(2):284-293.

Abstract

Reductionist in vitro model systems which mimic specific extracellular matrix functions in a highly controlled manner, termed artificial extracellular matrices (aECM), have increasingly been used to elucidate the role of cell-ECM interactions in regulating cell fate. To better understand the interplay of biophysical and biochemical effectors in controlling three-dimensional cell migration, a poly(ethylene glycol)-based aECM platform was used in this study to explore the influence of matrix cross-linking density, represented here by stiffness, on cell migration in vitro and in vivo. In vitro, the migration behavior of single preosteoblastic cells within hydrogels of varying stiffness and susceptibilities to degradation by matrix metalloproteases was assessed by time-lapse microscopy. Migration behavior was seen to be strongly dependent on matrix stiffness, with two regimes identified: a nonproteolytic migration mode dominating at relatively low matrix stiffness and proteolytic migration at higher stiffness. Subsequent in vivo experiments revealed a similar stiffness dependence of matrix remodeling, albeit less sensitive to the matrix metalloprotease sensitivity. Therefore, our aECM model system is well suited to unveil the role of biophysical and biochemical determinants of physiologically relevant cell migration phenomena.

Abstract

Reductionist in vitro model systems which mimic specific extracellular matrix functions in a highly controlled manner, termed artificial extracellular matrices (aECM), have increasingly been used to elucidate the role of cell-ECM interactions in regulating cell fate. To better understand the interplay of biophysical and biochemical effectors in controlling three-dimensional cell migration, a poly(ethylene glycol)-based aECM platform was used in this study to explore the influence of matrix cross-linking density, represented here by stiffness, on cell migration in vitro and in vivo. In vitro, the migration behavior of single preosteoblastic cells within hydrogels of varying stiffness and susceptibilities to degradation by matrix metalloproteases was assessed by time-lapse microscopy. Migration behavior was seen to be strongly dependent on matrix stiffness, with two regimes identified: a nonproteolytic migration mode dominating at relatively low matrix stiffness and proteolytic migration at higher stiffness. Subsequent in vivo experiments revealed a similar stiffness dependence of matrix remodeling, albeit less sensitive to the matrix metalloprotease sensitivity. Therefore, our aECM model system is well suited to unveil the role of biophysical and biochemical determinants of physiologically relevant cell migration phenomena.

Statistics

Citations

Dimensions.ai Metrics
137 citations in Web of Science®
137 citations in Scopus®
194 citations in Microsoft Academic
Google Scholar™

Altmetrics

Downloads

235 downloads since deposited on 21 Feb 2011
31 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
04 Faculty of Medicine > University Hospital Zurich > Clinic for Obstetrics
04 Faculty of Medicine > Center for Dental Medicine > Clinic for Cranio-Maxillofacial Surgery
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2011
Deposited On:21 Feb 2011 16:29
Last Modified:17 Feb 2018 13:15
Publisher:Elsevier
ISSN:0006-3495
OA Status:Hybrid
Free access at:PubMed ID. An embargo period may apply.
Publisher DOI:https://doi.org/10.1016/j.bpj.2010.11.082
PubMed ID:21244824
Project Information:
  • : FunderFP7
  • : Grant ID214402
  • : Project TitleANGIOSCAFF - Angiogenesis-inducing Bioactive and Bioresponsive Scaffolds in Tissue Enginering

Download

Download PDF  'Elucidating the role of matrix stiffness in 3D cell migration and remodeling'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 2MB
View at publisher