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Aetiology of bovine abortion in Switzerland from 1986 to 1995 - a retrospective study with emphasis on detection of neospora caninum and toxoplasma gondii by PCR


Reitt, K; Hilbe, M; Voegtlin, A; Corboz, L; Haessig, M; Pospischil, A (2007). Aetiology of bovine abortion in Switzerland from 1986 to 1995 - a retrospective study with emphasis on detection of neospora caninum and toxoplasma gondii by PCR. Journal of Veterinary Medicine Series A, 54(1):15-22.

Abstract

In a retrospective study, covering the period from 1986 to 1995, tissues of aborted fetuses were re-examined. A total of 347 cases were tested immunohistochemically, among them samples of 223 brains were examined for Neospora caninum, Toxoplasma gondii and Bovine Virus Diarrhoea Virus (BVDV), and 249 placentae for Chlamydiaceae. Two real-time PCR assays, one for N. caninum, and one for T. gondii, were developed. These potential abortion-inducing agents were detected – and confirmed by PCR, except for BVDV – in 16.1% (N. caninum), 0% (T. gondii), 9.9% (BVDV) and 0.8% (Chlamydiales) of the cases examined. Immunohistochemistry proved to be inadequate for the detection of the protozoal epitopes, whereas it was confirmed as a very useful tool for the detection of BVDV. In abortion material, PCR is considered to be more suitable for the detection of protozoa and Chlamydophila abortus, an adequate sampling presupposed.

Abstract

In a retrospective study, covering the period from 1986 to 1995, tissues of aborted fetuses were re-examined. A total of 347 cases were tested immunohistochemically, among them samples of 223 brains were examined for Neospora caninum, Toxoplasma gondii and Bovine Virus Diarrhoea Virus (BVDV), and 249 placentae for Chlamydiaceae. Two real-time PCR assays, one for N. caninum, and one for T. gondii, were developed. These potential abortion-inducing agents were detected – and confirmed by PCR, except for BVDV – in 16.1% (N. caninum), 0% (T. gondii), 9.9% (BVDV) and 0.8% (Chlamydiales) of the cases examined. Immunohistochemistry proved to be inadequate for the detection of the protozoal epitopes, whereas it was confirmed as a very useful tool for the detection of BVDV. In abortion material, PCR is considered to be more suitable for the detection of protozoa and Chlamydophila abortus, an adequate sampling presupposed.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Virology
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2007
Deposited On:25 Mar 2009 10:00
Last Modified:05 Apr 2016 12:31
Publisher:Wiley-Blackwell
ISSN:1865-1674
Publisher DOI:https://doi.org/10.1111/j.1439-0442.2007.00913.x
PubMed ID:17359449

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