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Construction of cis-regulatory input functions of yeast promoters


Ratna, P; Becskei, A (2011). Construction of cis-regulatory input functions of yeast promoters. In: Becskei, A. Yeast Genetic Networks : Methods and Protocols. New York [etc.]: Springer, 45-61.

Abstract

Promoters contain a large number of binding sites for transcriptional factors transmitting signals from a variety of cellular pathways. The promoter processes these input signals and sets the level of gene expression, the output of the gene. Here, we describe how to design genetic constructs and measure gene expression to deliver data suitable for quantitative analysis. Synthetic genetic constructs are well suited to precisely control and measure gene expression to construct cis-regulatory input functions. These functions can be used to predict gene expression based on signal intensities transmitted to activators and repressors in the gene regulatory region. Simple models of gene expression are presented for competitive and noncompetitive repressions. Complex phenomena, exemplified by synergistic silencing, are modeled by reaction-diffusion equations.

Abstract

Promoters contain a large number of binding sites for transcriptional factors transmitting signals from a variety of cellular pathways. The promoter processes these input signals and sets the level of gene expression, the output of the gene. Here, we describe how to design genetic constructs and measure gene expression to deliver data suitable for quantitative analysis. Synthetic genetic constructs are well suited to precisely control and measure gene expression to construct cis-regulatory input functions. These functions can be used to predict gene expression based on signal intensities transmitted to activators and repressors in the gene regulatory region. Simple models of gene expression are presented for competitive and noncompetitive repressions. Complex phenomena, exemplified by synergistic silencing, are modeled by reaction-diffusion equations.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2011
Deposited On:03 May 2011 12:32
Last Modified:05 Apr 2016 14:54
Publisher:Springer
Series Name:Methods in Molecular Biology
Number:734
ISSN:1064-3745 (P) 1940-6029 (E)
ISBN:978-1-61779-085-0
Publisher DOI:https://doi.org/10.1007/978-1-61779-086-7_3
PubMed ID:21468984

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