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Cellulose as an extra-cellular matrix component present in Enterobacter sakazakii biofilms


Grimm, M; Stephan, R; Iversen, C; Manzardo, G; Rattei, T; Riedel, K; Ruepp, A; Frishman, D; Lehner, A (2008). Cellulose as an extra-cellular matrix component present in Enterobacter sakazakii biofilms. Journal of Food Protection, 71(1):13-18.

Abstract

We report the identification and characterization of cellulose as an extracellular matrix component present in the biofilm of a E. sakazakii clinical isolate, after growth in nutrient deficient (M9) media. Using a bacterial artificial cloning (BAC) approach in E. coli, with subsequent screening of transformants for fluorescence on calcofluor plates, nine genes organized in two operons were identified as putatively responsible for the biosynthesis of cellulose. Among the genes already described for cellulose production in E. coli two more genes were identified, putatively transcribed together with the genes from the first operon. Putative cellulose in E. sakazakii ES5 biofilm grown on glass cover slips was visualized by calcofluor staining and confocal fluorescence laser scanning microscopy. For the first time, the presence of cellulose in biofilms produced by E. sakazakii was confirmed by methylation analysis.

Abstract

We report the identification and characterization of cellulose as an extracellular matrix component present in the biofilm of a E. sakazakii clinical isolate, after growth in nutrient deficient (M9) media. Using a bacterial artificial cloning (BAC) approach in E. coli, with subsequent screening of transformants for fluorescence on calcofluor plates, nine genes organized in two operons were identified as putatively responsible for the biosynthesis of cellulose. Among the genes already described for cellulose production in E. coli two more genes were identified, putatively transcribed together with the genes from the first operon. Putative cellulose in E. sakazakii ES5 biofilm grown on glass cover slips was visualized by calcofluor staining and confocal fluorescence laser scanning microscopy. For the first time, the presence of cellulose in biofilms produced by E. sakazakii was confirmed by methylation analysis.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:570 Life sciences; biology
580 Plants (Botany)
610 Medicine & health
Uncontrolled Keywords:Enterobacter sakazakii, cellulose containing biofilm, methylation analysis
Language:English
Date:January 2008
Deposited On:25 Nov 2008 13:32
Last Modified:05 Apr 2016 12:32
Publisher:International Association for Food Protection
ISSN:0362-028X
Official URL:http://www.ingentaconnect.com/content/iafp/jfp/2008/00000071/00000001/art00003
PubMed ID:18236657

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