Numerous zoonotic diseases cause morbidity, mortality and productivity losses in both humans and animal populations. Recent studies suggest that these diseases can produce large societal impacts in endemic areas. Estimates of monetary impact and disease burden provide essential, evidence-based data for conducting cost-benefit and cost-utility analyses that can contribute to securing political will and financial and technical resources. To evaluate burden, monetary and non-monetary impacts of zoonoses on human health, agriculture and society should be comprehensively considered. This chapter reviews the framework used to assess the health impact and burden of zoonoses and the data needed to estimate the extent of the problem for societies. Case studies are presented to illustrate the use of burden of disease assessment for the zoonotic diseases cystic echinococcosis, Taenia solium cysticercosis, brucellosis and rabies.