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Occurrence and genotypes of Campylobacter in broiler flocks, other farm animals, and the environment during several rearing periods on selected poultry farms.


Zweifel, C; Scheu, K D; Keel, M; Renggli, F; Stephan, R (2008). Occurrence and genotypes of Campylobacter in broiler flocks, other farm animals, and the environment during several rearing periods on selected poultry farms. International Journal of Food Microbiology, 125(2):182-187.

Abstract

On 15 poultry farms, broiler flocks, other farm animals, and the environment were examined for Campylobacter. Flocks were examined weekly for six or three rearing periods. Of the 5’154 collected samples, 311 (6%) from 14 farms were Campylobacter positive. Positive samples originated from broiler flocks, the broiler houses, cattle, pigs, bantams, a horse, a laying hen flock, and a mouse. Amongst them, 288 tested positive for C. jejuni and 92 for C. coli. The analysis of 917 isolates by flagellin gene typing and pulsed-field gel electrophoresis, and of 15 assorted strains by amplified fragment length polymorphism allowed the following conclusions: (i) on eight farms (A, D, H, K, L, M, O, P) identical genotypes were isolated from broilers and other farm animals emphasizing their importance as reservoirs and risk factors for flock colonization and retrieving the role of personnel moving between areas as potential vectors; (ii) on four farms (C, D, I, L), indications of persistent contamination of the broiler house were evident and thereby the importance of efficient cleaning and disinfection was underlined; (iii) the previously described sources for broiler flock colonization could be excluded for certain genotypes from eight farms suggesting the existence of more potential vectors or niches; (iv) especially on farms with extensive outdoor flocks, multiple genotypes were found within a rearing period; and (v) some genotypes were identical across farms. The significance of such strains remains to be elucidated.

Abstract

On 15 poultry farms, broiler flocks, other farm animals, and the environment were examined for Campylobacter. Flocks were examined weekly for six or three rearing periods. Of the 5’154 collected samples, 311 (6%) from 14 farms were Campylobacter positive. Positive samples originated from broiler flocks, the broiler houses, cattle, pigs, bantams, a horse, a laying hen flock, and a mouse. Amongst them, 288 tested positive for C. jejuni and 92 for C. coli. The analysis of 917 isolates by flagellin gene typing and pulsed-field gel electrophoresis, and of 15 assorted strains by amplified fragment length polymorphism allowed the following conclusions: (i) on eight farms (A, D, H, K, L, M, O, P) identical genotypes were isolated from broilers and other farm animals emphasizing their importance as reservoirs and risk factors for flock colonization and retrieving the role of personnel moving between areas as potential vectors; (ii) on four farms (C, D, I, L), indications of persistent contamination of the broiler house were evident and thereby the importance of efficient cleaning and disinfection was underlined; (iii) the previously described sources for broiler flock colonization could be excluded for certain genotypes from eight farms suggesting the existence of more potential vectors or niches; (iv) especially on farms with extensive outdoor flocks, multiple genotypes were found within a rearing period; and (v) some genotypes were identical across farms. The significance of such strains remains to be elucidated.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Uncontrolled Keywords:Campylobacter; Broiler flocks; Farm animals; Environment; Genotyping
Language:English
Date:15 July 2008
Deposited On:12 Nov 2008 09:26
Last Modified:17 Dec 2017 06:14
Publisher:Elsevier
ISSN:0168-1605
Publisher DOI:https://doi.org/10.1016/j.ijfoodmicro.2008.03.038

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