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Characterization of nuclear microsatellite loci in the calcareous fen specialist Scorpidium cossonii (Calliergonaceae)


Kophimai, Y; Cornejo, C; Peintinger, M; Bergamini, A; Scheidegger, C (2011). Characterization of nuclear microsatellite loci in the calcareous fen specialist Scorpidium cossonii (Calliergonaceae). American Journal of Botany, 98(10):e290-e292.

Abstract

Premise of the study: Nuclear microsatellite markers were developed in the fen specialist moss, Scorpidium cossonii, to study genetic diversity and genetic structure of this species in relation to land-use types, habitat fragmentation, and habitat conservation measures. Methods and Results: The polymorphisms of 14 microsatellite markers were characterized. All primers were tested on 140 gametophytes collected from four populations in Switzerland. The primers amplified di- and trinucleotide repeats with three to 26 alleles per locus. The primers worked also in the two other species of the genus: 14 and 12 primers successfully amplified in S. revolvens and S. scorpioides, respectively. Conclusions: In future studies, our primers have the potential to provide valuable information on genetic diversity, genetic structure, and on historical and recent gene flow in S. cossonii; they should also enable related research in other Scorpidium species.

Abstract

Premise of the study: Nuclear microsatellite markers were developed in the fen specialist moss, Scorpidium cossonii, to study genetic diversity and genetic structure of this species in relation to land-use types, habitat fragmentation, and habitat conservation measures. Methods and Results: The polymorphisms of 14 microsatellite markers were characterized. All primers were tested on 140 gametophytes collected from four populations in Switzerland. The primers amplified di- and trinucleotide repeats with three to 26 alleles per locus. The primers worked also in the two other species of the genus: 14 and 12 primers successfully amplified in S. revolvens and S. scorpioides, respectively. Conclusions: In future studies, our primers have the potential to provide valuable information on genetic diversity, genetic structure, and on historical and recent gene flow in S. cossonii; they should also enable related research in other Scorpidium species.

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Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
Uncontrolled Keywords:bryophytes, calcareous fens, cross-species amplification, microsatellites, Scorpidium cossonii
Language:English
Date:2011
Deposited On:20 Jan 2012 15:24
Last Modified:20 Dec 2017 00:10
Publisher:Botanical Society of America
ISSN:0002-9122
Publisher DOI:https://doi.org/10.3732/ajb.1100144
PubMed ID:21926311

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