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Triiodothyronine stimulates glucose transport in bone cells


Zoidis, E; Ghirlanda-Keller, C; Schmid, C (2012). Triiodothyronine stimulates glucose transport in bone cells. Endocrine, 41(3):501-511.

Abstract

Thyroid hormones increase energy expenditure and bone turnover in vivo. To study whether 3,3',5-triiodo-l-thyronine (T(3)) stimulates the uptake of glucose in osteoblastic cells, PyMS (a cell line derived from rat bone) cells were kept in serum-free culture medium and treated with T(3). We measured [1-(14)C]-2-deoxy-D: -glucose (2DG) uptake and looked for expression of the high-affinity glucose transporters GLUT1 and GLUT3 by northern and western analysis. T(3) did not influence the cell number but slightly (1.3-fold) increased the protein content of the cell cultures. 2DG uptake was low in serum-deprived cell cultures and was increased by T(3) (up to 2.5-fold at 1 nmol l(-1) after 4 days) in a dose- and time-dependent manner. Triiodothyronine at 1 nmol l(-1) increased GLUT1 and GLUT3 abundance in membranes. Therefore, increased glucose uptake induced by T(3) in osteoblasts may be mediated by the known high-affinity glucose transporters GLUT1 and GLUT3.

Abstract

Thyroid hormones increase energy expenditure and bone turnover in vivo. To study whether 3,3',5-triiodo-l-thyronine (T(3)) stimulates the uptake of glucose in osteoblastic cells, PyMS (a cell line derived from rat bone) cells were kept in serum-free culture medium and treated with T(3). We measured [1-(14)C]-2-deoxy-D: -glucose (2DG) uptake and looked for expression of the high-affinity glucose transporters GLUT1 and GLUT3 by northern and western analysis. T(3) did not influence the cell number but slightly (1.3-fold) increased the protein content of the cell cultures. 2DG uptake was low in serum-deprived cell cultures and was increased by T(3) (up to 2.5-fold at 1 nmol l(-1) after 4 days) in a dose- and time-dependent manner. Triiodothyronine at 1 nmol l(-1) increased GLUT1 and GLUT3 abundance in membranes. Therefore, increased glucose uptake induced by T(3) in osteoblasts may be mediated by the known high-affinity glucose transporters GLUT1 and GLUT3.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Endocrinology and Diabetology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2012
Deposited On:02 Apr 2012 07:23
Last Modified:07 Dec 2017 11:43
Publisher:Springer
ISSN:1355-008X
Publisher DOI:https://doi.org/10.1007/s12020-012-9594-2
PubMed ID:22258767

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