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Polymorphic microsatellite loci for the endangered pine hoverfly Blera fallax (Diptera: Syrphidae)


Rotheray, Ellen L; Greminger, Maja P; Nater, Alexander; Krützen, Michael; Goulson, David; Bussière, Luc F (2012). Polymorphic microsatellite loci for the endangered pine hoverfly Blera fallax (Diptera: Syrphidae). Conservation Genetics Resources, 4(1):117-120.

Abstract

We describe eleven polymorphic microsatellite loci developed for Blera fallax using ‘next generation’ 454 whole genome shotgun sequencing, along with conditions for three multiplex PCR reactions. We tested allelic variation on forty B. fallax individuals from Scotland and Sweden. Allelic richness and expected heterozygosity were 3.03 (±0.274) and 0.391 (±0.057) respectively. The number of alleles per locus ranged from 2 to 6. We anticipate that these loci will assist conservation management by allowing the monitoring of translocated populations, estimating effective population size, and assessing population structure and dispersal in Scotland and across Europe.

Abstract

We describe eleven polymorphic microsatellite loci developed for Blera fallax using ‘next generation’ 454 whole genome shotgun sequencing, along with conditions for three multiplex PCR reactions. We tested allelic variation on forty B. fallax individuals from Scotland and Sweden. Allelic richness and expected heterozygosity were 3.03 (±0.274) and 0.391 (±0.057) respectively. The number of alleles per locus ranged from 2 to 6. We anticipate that these loci will assist conservation management by allowing the monitoring of translocated populations, estimating effective population size, and assessing population structure and dispersal in Scotland and across Europe.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Anthropology
Dewey Decimal Classification:300 Social sciences, sociology & anthropology
Language:English
Date:2012
Deposited On:10 Apr 2012 06:48
Last Modified:07 Dec 2017 13:14
Publisher:Springer
ISSN:1877-7252
Publisher DOI:https://doi.org/10.1007/s12686-011-9488-2

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