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mRNA profiling for body fluid identification


Haas, C; Klesser, B; Kratzer, A; Bär, W (2008). mRNA profiling for body fluid identification. Forensic Science International: Genetics Supplement Series, 1(1):37-38.

Abstract

mRNA profiling is a promising new method for the identification of body fluids from biological stains. A multiplex reverse transcription endpoint PCR method was adapted for the identification of blood, saliva, semen, vaginal secretions and menstrual blood. For specificity, sensitivity and suitability to biological stains the endpoint PCR method was satisfying. Up to 15 months old stains were shown to be suitable for mRNA profiling. In addition a realtime PCR assay was tested for sensitivity with blood and semen samples, providing comparable results to endpoint PCR or enzymatic tests.

Abstract

mRNA profiling is a promising new method for the identification of body fluids from biological stains. A multiplex reverse transcription endpoint PCR method was adapted for the identification of blood, saliva, semen, vaginal secretions and menstrual blood. For specificity, sensitivity and suitability to biological stains the endpoint PCR method was satisfying. Up to 15 months old stains were shown to be suitable for mRNA profiling. In addition a realtime PCR assay was tested for sensitivity with blood and semen samples, providing comparable results to endpoint PCR or enzymatic tests.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Legal Medicine
Dewey Decimal Classification:340 Law
610 Medicine & health
Language:English
Date:2008
Deposited On:24 Nov 2008 16:05
Last Modified:22 Nov 2017 20:09
Publisher:Elsevier
ISSN:1875-175X
Publisher DOI:https://doi.org/10.1016/j.fsigss.2007.10.064

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