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Electron microscopy


Luder, H U; Amstad-Jossi, M (2012). Electron microscopy. In: Kioussi, C. Odontogenesis. Methods and Protocols. New York: Humana Press, 81-93.

Abstract

Correlative light (LM) and transmission electron microscopic (TEM) analysis is useful, if ultrastructural details of cells need to be related to functional aspects which can only be examined at the LM level. The first protocol presented here introduces a relatively simple way of obtaining TEM images which, on the one hand, reveal ultrastructural details of individual cells and, on the other hand, are large enough to allow a correlation with light micrographs. The second protocol describes a technique for estimating mineral densities of hard tissues using backscattered electron images obtained with a scanning electron microscope. This technique can be used to analyze the mineralization processes which occur throughout tooth formation.

Abstract

Correlative light (LM) and transmission electron microscopic (TEM) analysis is useful, if ultrastructural details of cells need to be related to functional aspects which can only be examined at the LM level. The first protocol presented here introduces a relatively simple way of obtaining TEM images which, on the one hand, reveal ultrastructural details of individual cells and, on the other hand, are large enough to allow a correlation with light micrographs. The second protocol describes a technique for estimating mineral densities of hard tissues using backscattered electron images obtained with a scanning electron microscope. This technique can be used to analyze the mineralization processes which occur throughout tooth formation.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Institute of Oral Biology
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2012
Deposited On:07 Jun 2012 09:14
Last Modified:07 Dec 2017 14:18
Publisher:Humana Press
Series Name:Methods in Molecular Biology
Number:887
ISSN:1064-3745
ISBN:978-1-61779-859-7
Publisher DOI:https://doi.org/10.1007/978-1-61779-860-3_9
PubMed ID:22566049

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