Header

UZH-Logo

Maintenance Infos

Rapid selection of high-affinity binders using ribosome display


Dreier, Birgit; Plückthun, Andreas (2012). Rapid selection of high-affinity binders using ribosome display. Methods in Molecular Biology, 805:261-286.

Abstract

Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affinity binders from libraries of folded proteins. It has been successfully applied to single-chain Fv fragments of antibodies and alternative scaffolds, such as Designed Ankyrin Repeat Proteins (DARPins). High-affinity binders with new target specificity can be obtained from highly diverse DARPin libraries in only a few selection rounds. In this protocol, the selection from the library and the process of affinity maturation and off-rate selection are explained in detail.

Abstract

Ribosome display has proven to be a powerful in vitro selection and evolution method for generating high-affinity binders from libraries of folded proteins. It has been successfully applied to single-chain Fv fragments of antibodies and alternative scaffolds, such as Designed Ankyrin Repeat Proteins (DARPins). High-affinity binders with new target specificity can be obtained from highly diverse DARPin libraries in only a few selection rounds. In this protocol, the selection from the library and the process of affinity maturation and off-rate selection are explained in detail.

Statistics

Citations

21 citations in Web of Science®
20 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

0 downloads since deposited on 11 Oct 2012
0 downloads since 12 months

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Department of Biochemistry
07 Faculty of Science > Department of Biochemistry
Dewey Decimal Classification:570 Life sciences; biology
Language:English
Date:2012
Deposited On:11 Oct 2012 08:56
Last Modified:21 Nov 2017 16:10
Publisher:Springer
ISSN:1064-3745
Publisher DOI:https://doi.org/10.1007/978-1-61779-379-0_15
PubMed ID:22094811

Download

Content: Published Version
Language: English
Filetype: PDF - Registered users only
Size: 303kB
View at publisher