Header

UZH-Logo

Maintenance Infos

Myoblasts inhibit prostate cancer growth by paracrine secretion of TNF alpha


Stölting, Meline N L; Ferrari, Stefano; Handschin, Christoph; Becskei, Attila; Provenzano, Maurizio; Sulser, Tullio; Eberli, Daniel (2013). Myoblasts inhibit prostate cancer growth by paracrine secretion of TNF alpha. Journal of Urology, 189(5):1952-1959.

Abstract

PURPOSE: Myoblasts are capable of forming muscle fibers after transplantation and are therefore envisioned as a treatment for urinary incontinence after radical prostatectomy. However, the safety of this treatment and the interaction of myoblasts with remaining neighboring cancerare unknown. We investigated the interactions between myoblasts and prostate carcinoma cellsin vitro and in vivo. MATERIALS AND METHODS: Myoblasts isolated fromrectus abdominis were used in a series of co-culture experiments with prostate cancer cells and subcutaneously co-injected in vivo. Cell proliferation, cell cycle arrest and apoptosis of cancers in co-culture with myoblasts were assessed. Tumor volume and metastasis formation were evaluated in a mouse model. Tissue specific markers were assessed by immunohistochemistry, FACS analyses, Western blot and RT-qPCR. RESULTS: In this study we have demonstrated that myoblasts, in proximity to tumor, provide paracrine TNFα to their microenvironment, decreasing tumor growth of all prostate cancer cell lines examined. Co-culture experiments showed induction of cell cycle arrest, tumor death by apoptosis and increased differentiation of myoblasts. This effect was largely blocked by TNFα inhibition. The same outcome was demonstrated in a mouse model, where co-injected human myoblasts also inhibited tumor growth and metastasis formation of all prostate cancer cell lines evaluated. CONCLUSIONS: Myoblasts restrict prostate cancer growth and limit metastasis formation by paracrine TNFα secretion in vitro and in vivo.

Abstract

PURPOSE: Myoblasts are capable of forming muscle fibers after transplantation and are therefore envisioned as a treatment for urinary incontinence after radical prostatectomy. However, the safety of this treatment and the interaction of myoblasts with remaining neighboring cancerare unknown. We investigated the interactions between myoblasts and prostate carcinoma cellsin vitro and in vivo. MATERIALS AND METHODS: Myoblasts isolated fromrectus abdominis were used in a series of co-culture experiments with prostate cancer cells and subcutaneously co-injected in vivo. Cell proliferation, cell cycle arrest and apoptosis of cancers in co-culture with myoblasts were assessed. Tumor volume and metastasis formation were evaluated in a mouse model. Tissue specific markers were assessed by immunohistochemistry, FACS analyses, Western blot and RT-qPCR. RESULTS: In this study we have demonstrated that myoblasts, in proximity to tumor, provide paracrine TNFα to their microenvironment, decreasing tumor growth of all prostate cancer cell lines examined. Co-culture experiments showed induction of cell cycle arrest, tumor death by apoptosis and increased differentiation of myoblasts. This effect was largely blocked by TNFα inhibition. The same outcome was demonstrated in a mouse model, where co-injected human myoblasts also inhibited tumor growth and metastasis formation of all prostate cancer cell lines evaluated. CONCLUSIONS: Myoblasts restrict prostate cancer growth and limit metastasis formation by paracrine TNFα secretion in vitro and in vivo.

Statistics

Citations

6 citations in Web of Science®
7 citations in Scopus®
Google Scholar™

Altmetrics

Downloads

137 downloads since deposited on 14 Dec 2012
30 downloads since 12 months
Detailed statistics

Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Molecular Cancer Research
07 Faculty of Science > Institute of Molecular Cancer Research

04 Faculty of Medicine > University Hospital Zurich > Urological Clinic
04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2013
Deposited On:14 Dec 2012 07:38
Last Modified:07 Dec 2017 17:26
Publisher:Elsevier
ISSN:0022-5347
Publisher DOI:https://doi.org/10.1016/j.juro.2012.10.071
PubMed ID:23123370

Download

Download PDF  'Myoblasts inhibit prostate cancer growth by paracrine secretion of TNF alpha'.
Preview
Content: Accepted Version
Filetype: PDF
Size: 745kB
View at publisher