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Effect of cerium chloride application on fibroblast and osteoblast proliferation and differentiation


Schmidlin, Patrick R; Tchouboukov, Alexandre; Wegehaupt, Florian J; Weber, Franz E (2012). Effect of cerium chloride application on fibroblast and osteoblast proliferation and differentiation. Archives of Oral Biology, 57(7):892-897.

Abstract

OBJECTIVE: This study investigated effects of cerium-chloride on fibroblast and osteoblast differentiation and proliferation.
METHODS: MC3T3-E1 cells were plated for an alkaline phosphatase (ALP) activity test. On day 3, CeCl(3)-solutions (1, 5 or 10, w/v%) were added. After 10s, the solutions were aspirated and washed to remove residual CeCl(3). On day 6 ALP activity was determined. Cell activity and proliferation was assessed by thiazolyl blue tetrazolium dye reduction assay (MTT-test) also 3 days after exposure to the CeCl(3)-solutions. Calcium deposition by preosteoblastic cells was determined 4 weeks after the exposure of the cells by alizarin red staining. Furthermore, in all experiments the influence of adding rhBMP-2 was tested. Statistical analysis was performed by repeated-measures ANOVA using the post hoc Fisher least significant difference (LSD) test. Statistical significance was set at p < 0.05.
RESULTS: Exposure to a Ce-solution of 1% or higher reduced ALP activity significantly. The addition of rhBMP-2 was able to elevate ALP activity above control level. MTT-test showed a significant decrease in cellular activity by 5% Ce or higher. The addition of rhBMP-2 had no positive effect. For human foreskin fibroblasts, exposure to even 10% Ce yielded a significant increase in cellular activity. Ce reduced calcium deposition to a level of below 50% of the control. The addition of rhBMP-2 restored mineral deposition to control levels for all Ce concentrations.
CONCLUSION: CeCl(3) had a stimulating effect on fibroblasts but a depressing influence on osteoblasts. However, adding rhBMP-2 could compensate the latter influence.

Abstract

OBJECTIVE: This study investigated effects of cerium-chloride on fibroblast and osteoblast differentiation and proliferation.
METHODS: MC3T3-E1 cells were plated for an alkaline phosphatase (ALP) activity test. On day 3, CeCl(3)-solutions (1, 5 or 10, w/v%) were added. After 10s, the solutions were aspirated and washed to remove residual CeCl(3). On day 6 ALP activity was determined. Cell activity and proliferation was assessed by thiazolyl blue tetrazolium dye reduction assay (MTT-test) also 3 days after exposure to the CeCl(3)-solutions. Calcium deposition by preosteoblastic cells was determined 4 weeks after the exposure of the cells by alizarin red staining. Furthermore, in all experiments the influence of adding rhBMP-2 was tested. Statistical analysis was performed by repeated-measures ANOVA using the post hoc Fisher least significant difference (LSD) test. Statistical significance was set at p < 0.05.
RESULTS: Exposure to a Ce-solution of 1% or higher reduced ALP activity significantly. The addition of rhBMP-2 was able to elevate ALP activity above control level. MTT-test showed a significant decrease in cellular activity by 5% Ce or higher. The addition of rhBMP-2 had no positive effect. For human foreskin fibroblasts, exposure to even 10% Ce yielded a significant increase in cellular activity. Ce reduced calcium deposition to a level of below 50% of the control. The addition of rhBMP-2 restored mineral deposition to control levels for all Ce concentrations.
CONCLUSION: CeCl(3) had a stimulating effect on fibroblasts but a depressing influence on osteoblasts. However, adding rhBMP-2 could compensate the latter influence.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Dental Medicine > Clinic for Preventive Dentistry, Periodontology and Cariology
04 Faculty of Medicine > Center for Dental Medicine > Clinic for Cranio-Maxillofacial Surgery
04 Faculty of Medicine > University Hospital Zurich > Division of Surgical Research
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:July 2012
Deposited On:10 Jan 2013 11:17
Last Modified:07 Dec 2017 17:39
Publisher:Pergamon
ISSN:0003-9969
Publisher DOI:https://doi.org/10.1016/j.archoralbio.2012.01.010
PubMed ID:22325621

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