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DOCA sensitive pendrin expression in kidney, heart, lung and thyroid tissues


Pelzl, Lisann; Pakladok, Tatsiana; Pathare, Ganesh; Fakhri, Hajar; Michael, Diana; Wagner, Carsten A; Paulmichl, Markus; Lang, Florian (2012). DOCA sensitive pendrin expression in kidney, heart, lung and thyroid tissues. Cellular Physiology and Biochemistry, 30(6):1491-1501.

Abstract

BACKGROUND/AIMS: Pendrin (SLC26A4), a transporter accomplishing anion exchange, is expressed in inner ear, thyroid gland, kidneys, lung, liver and heart. Loss or reduction of function mutations of SLC26A4 underlie Pendred syndrome, a disorder invariably leading to hearing loss with enlarged vestibular aqueducts and in some patients to hypothyroidism and goiter. Renal pendrin expression is up-regulated by mineralocorticoids such as aldosterone or deoxycorticosterone (DOCA). Little is known about the impact of mineralocorticoids on pendrin expression in extrarenal tissues.
METHODS: The present study utilized RT-qPCR and Western blotting to quantify the transcript levels and protein abundance of Slc26a4 in murine kidney, thyroid, heart and lung prior to and following subcutaneous administration of 100 mg/kg DOCA.
RESULTS: Slc26a4 transcript levels as compared to Gapdh transcript levels were significantly increased by DOCA treatment in kidney, heart, lung and thyroid. Accordingly pendrin protein expression was again significantly increased by DOCA treatment in kidney, heart, lung and thyroid.
CONCLUSION: The observations reveal mineralocorticoid sensitivity of pendrin expression in kidney, heart, thyroid and lung

Abstract

BACKGROUND/AIMS: Pendrin (SLC26A4), a transporter accomplishing anion exchange, is expressed in inner ear, thyroid gland, kidneys, lung, liver and heart. Loss or reduction of function mutations of SLC26A4 underlie Pendred syndrome, a disorder invariably leading to hearing loss with enlarged vestibular aqueducts and in some patients to hypothyroidism and goiter. Renal pendrin expression is up-regulated by mineralocorticoids such as aldosterone or deoxycorticosterone (DOCA). Little is known about the impact of mineralocorticoids on pendrin expression in extrarenal tissues.
METHODS: The present study utilized RT-qPCR and Western blotting to quantify the transcript levels and protein abundance of Slc26a4 in murine kidney, thyroid, heart and lung prior to and following subcutaneous administration of 100 mg/kg DOCA.
RESULTS: Slc26a4 transcript levels as compared to Gapdh transcript levels were significantly increased by DOCA treatment in kidney, heart, lung and thyroid. Accordingly pendrin protein expression was again significantly increased by DOCA treatment in kidney, heart, lung and thyroid.
CONCLUSION: The observations reveal mineralocorticoid sensitivity of pendrin expression in kidney, heart, thyroid and lung

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Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Physiology
07 Faculty of Science > Institute of Physiology

04 Faculty of Medicine > Center for Integrative Human Physiology
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Uncontrolled Keywords:Slc26a4, Acidosis, Alkalosis, Liver, Kidney
Language:English
Date:2012
Deposited On:10 Jan 2013 11:39
Last Modified:28 Aug 2017 15:06
Publisher:Karger
ISSN:1015-8987
Additional Information:The final, published version of this article is available at http://www.karger.com/?doi=10.1159/000343337.
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1159/000343337
PubMed ID:23235354

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