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Analysis of optokinetic response in zebrafish by computer-based eye tracking


Huber-Reggi, Sabina P; Mueller, Kaspar P; Neuhauss, Stephan C F (2013). Analysis of optokinetic response in zebrafish by computer-based eye tracking. In: Weber, Bernhard H F; Langmann, Thomas. Retinal Degeneration. Methods and Protocols. Totowa: Humana Press, 139-160.

Abstract

Large-field movements in the visual surround trigger spontaneous, compensatory eye movements known as optokinetic response (OKR) in all vertebrates. In zebrafish (Danio rerio) the OKR is well developed at 5 days post fertilization and can be used in the laboratory for screening of visual performance following genetic manipulations or pharmaceutical treatments. Several setups for measurement of the zebrafish OKR have been described. All of them are based on the presentation of moving gratings to the larva or to the adult fish. However, they differ in the way of presenting gratings and in the method of analysis. Here, we describe a detailed protocol for our newest software that enables computer-generation of the moving stripes and automatic tracking of eye movement. This protocol makes it possible to quantitatively measure OKR in both larvae and adult fishes in a fast and reliable way.

Abstract

Large-field movements in the visual surround trigger spontaneous, compensatory eye movements known as optokinetic response (OKR) in all vertebrates. In zebrafish (Danio rerio) the OKR is well developed at 5 days post fertilization and can be used in the laboratory for screening of visual performance following genetic manipulations or pharmaceutical treatments. Several setups for measurement of the zebrafish OKR have been described. All of them are based on the presentation of moving gratings to the larva or to the adult fish. However, they differ in the way of presenting gratings and in the method of analysis. Here, we describe a detailed protocol for our newest software that enables computer-generation of the moving stripes and automatic tracking of eye movement. This protocol makes it possible to quantitatively measure OKR in both larvae and adult fishes in a fast and reliable way.

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Additional indexing

Item Type:Book Section, refereed, original work
Communities & Collections:04 Faculty of Medicine > Center for Integrative Human Physiology
07 Faculty of Science > Institute of Molecular Life Sciences
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Uncontrolled Keywords:Zebra fish larvae , Adult zebra fish, Optokinetic response , Eye movements, Vision , Visual behavior testing , Oculomotor
Language:English
Date:2013
Deposited On:19 Mar 2013 14:33
Last Modified:07 Dec 2017 17:57
Publisher:Humana Press
Series Name:Methods in Molecular Biology
Number:935
ISSN:1064-3745
ISBN:978-1-62703-079-3
Publisher DOI:https://doi.org/10.1007/978-1-62703-080-9_10
Related URLs:http://www.recherche-portal.ch/primo_library/libweb/action/search.do?fn=search&mode=Advanced&vid=ZAD&vl%28186672378UI0%29=isbn&vl%281UI0%29=contains&vl%28freeText0%29=978-1-62703-079-3
PubMed ID:23150366

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