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The MTT assay is a rapid and reliable quantitative method to assess Staphylococcus aureus induced endothelial cell damage


Seidl, Kati; Zinkernagel, Annelies S (2013). The MTT assay is a rapid and reliable quantitative method to assess Staphylococcus aureus induced endothelial cell damage. Journal of Microbiological Methods, 92(3):307-309.

Abstract

We established the (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay to assess damage induced by Staphylococcus aureus in human umbilical vein endothelial cells (HUVECs). The MTT assay was comparable to the previously published (51)chromium release assay. MTT reduction by intracellular S. aureus was negligible and therefore permits assessment of S. aureus induced EC damage.

Abstract

We established the (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assay to assess damage induced by Staphylococcus aureus in human umbilical vein endothelial cells (HUVECs). The MTT assay was comparable to the previously published (51)chromium release assay. MTT reduction by intracellular S. aureus was negligible and therefore permits assessment of S. aureus induced EC damage.

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18 citations in Web of Science®
18 citations in Scopus®
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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > University Hospital Zurich > Clinic for Infectious Diseases
Dewey Decimal Classification:610 Medicine & health
Language:English
Date:2013
Deposited On:24 Jan 2013 09:53
Last Modified:07 Dec 2017 18:27
Publisher:Elsevier
ISSN:0167-7012
Publisher DOI:https://doi.org/10.1016/j.mimet.2012.12.018
PubMed ID:23275136

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