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Developing hyperpolarized 13C spectroscopy and imaging for metabolic studies in the isolated perfused rat heart


Weiss, Kilian; Mariotti, Erika; Hill, Deborah K; Orton, Matthew R; Dunn, Joel T; Medina, Rodolfo A; Southworth, Richard; Kozerke, Sebastian; Eykyn, Thomas R (2012). Developing hyperpolarized 13C spectroscopy and imaging for metabolic studies in the isolated perfused rat heart. Applied Magnetic Resonance, 43(1-2):275-288.

Abstract

Hyperpolarized 13C magnetic resonance is a powerful tool for the study of cardiac metabolism. In this work, we have implemented protocols for the real-time hyperpolarized 13C investigation of Langendorff-perfused rat hearts using both non-selective non-localized spectroscopy and fast spectroscopic imaging. Following [1-13C] pyruvate infusion, we observed both catabolic and anaplerotic metabolic processes resulting in a number of metabolites, including bicarbonate, carbon dioxide, lactate, alanine and aspartate. Employing fast spectroscopic imaging, we were able to observe regional variations in pyruvate perfusion as well as in lactate and bicarbonate production.

Abstract

Hyperpolarized 13C magnetic resonance is a powerful tool for the study of cardiac metabolism. In this work, we have implemented protocols for the real-time hyperpolarized 13C investigation of Langendorff-perfused rat hearts using both non-selective non-localized spectroscopy and fast spectroscopic imaging. Following [1-13C] pyruvate infusion, we observed both catabolic and anaplerotic metabolic processes resulting in a number of metabolites, including bicarbonate, carbon dioxide, lactate, alanine and aspartate. Employing fast spectroscopic imaging, we were able to observe regional variations in pyruvate perfusion as well as in lactate and bicarbonate production.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:04 Faculty of Medicine > Institute of Biomedical Engineering
Dewey Decimal Classification:170 Ethics
610 Medicine & health
Language:English
Date:2012
Deposited On:14 Feb 2013 10:02
Last Modified:05 Apr 2016 16:26
Publisher:Springer Wien
ISSN:0937-9347
Publisher DOI:https://doi.org/10.1007/s00723-012-0349-2

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