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Phosphatidylcholine biosynthesis at low temperature: differential expression of CTP:phosphorylcholine cytidylyltransferase in Arabidopsis thaliana


Inatsugi, Rie; Nakamura, Masanobu; Nishida, Ikuo (2002). Phosphatidylcholine biosynthesis at low temperature: differential expression of CTP:phosphorylcholine cytidylyltransferase in Arabidopsis thaliana. Plant & Cell Physiology, 43(11):1342-1350.

Abstract

We cloned the gene and a cDNA for a second CTP: phosphorylcholine cytidylyltransferase (CCT, EC 2.7.7.15) annotated in chromosome 4 by the Arabidopsis genome project, and designated the gene AtCCT2 to discriminate it from the isogene AtCCT1 in chromosome 2. When Arabi- dopsis plants were chilled at 2°C for 12 h, the level of AtCCT2 transcripts in the rosettes increased about 6-fold over that before 2°C treatment. By contrast, no significant change occurred in the level of AtCCT1 transcripts during 7 d of 2°C treatment. Immunoblot analysis revealed that the level of AtCCT2 in the rosettes chilled at 2°C increased, and that the level of AtCCT1 showed minor changes, when compared with those before cold treatment. Total CCT activity measured at 2°C increased in plants subjected to 2°C treatment, and this increase was sufficient to account for lipid changes induced by the 2°C treatment. We there- fore concluded that Arabidopsis utilizes two distinct CCT isozymes for CDP-choline synthesis during cold acclima- tion. Our findings are important in understanding the physiological functions of CCT isozymes in Arabidopsis and will also stimulate efforts to understand the physiological significance of phosphatidylcholine at low temperatures.

Abstract

We cloned the gene and a cDNA for a second CTP: phosphorylcholine cytidylyltransferase (CCT, EC 2.7.7.15) annotated in chromosome 4 by the Arabidopsis genome project, and designated the gene AtCCT2 to discriminate it from the isogene AtCCT1 in chromosome 2. When Arabi- dopsis plants were chilled at 2°C for 12 h, the level of AtCCT2 transcripts in the rosettes increased about 6-fold over that before 2°C treatment. By contrast, no significant change occurred in the level of AtCCT1 transcripts during 7 d of 2°C treatment. Immunoblot analysis revealed that the level of AtCCT2 in the rosettes chilled at 2°C increased, and that the level of AtCCT1 showed minor changes, when compared with those before cold treatment. Total CCT activity measured at 2°C increased in plants subjected to 2°C treatment, and this increase was sufficient to account for lipid changes induced by the 2°C treatment. We there- fore concluded that Arabidopsis utilizes two distinct CCT isozymes for CDP-choline synthesis during cold acclima- tion. Our findings are important in understanding the physiological functions of CCT isozymes in Arabidopsis and will also stimulate efforts to understand the physiological significance of phosphatidylcholine at low temperatures.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
580 Plants (Botany)
Uncontrolled Keywords:Arabidopsis — Cold acclimation — CRT/DRE motif — CTP:phosphorylcholine cytidylyltransferase (EC 2.7.7.15) — Phosphatidylcholine.
Language:English
Date:2002
Deposited On:18 Feb 2013 12:22
Last Modified:05 Apr 2016 16:31
Publisher:Oxford University Press
ISSN:0032-0781
Publisher DOI:https://doi.org/10.1093/pcp/pcf169
PubMed ID:12461134

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