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Cell-specific expression profiling of rare cell types as exemplified by its impact on our understanding of female gametophyte development


Wuest, Samuel E; Schmid, Marc W; Grossniklaus, Ueli (2013). Cell-specific expression profiling of rare cell types as exemplified by its impact on our understanding of female gametophyte development. Current Opinion in Plant Biology, 16(1):41-49.

Abstract

Expression profiling of single cells can yield insights into cell specification, cellular differentiation processes, and cell type-specific responses to environmental stimuli. Recent work has established excellent tools to perform genome-wide expression studies of individual cell types, even if the cells of interest occur at low frequency within an organ. We review the advances and impact of gene expression studies of rare cell types, as exemplified by recently gained insights into the development and function of the angiosperm female gametophyte. The detailed transcriptional characterization of different stages during female gametophyte development has significantly helped to improve our understanding of cellular specification or cell-cell communication processes. Next-generation sequencing approaches-used increasingly for expression profiling-will now allow for comparative approaches that focus on agriculturally, ecologically or evolutionarily relevant aspects of plant reproduction.

Abstract

Expression profiling of single cells can yield insights into cell specification, cellular differentiation processes, and cell type-specific responses to environmental stimuli. Recent work has established excellent tools to perform genome-wide expression studies of individual cell types, even if the cells of interest occur at low frequency within an organ. We review the advances and impact of gene expression studies of rare cell types, as exemplified by recently gained insights into the development and function of the angiosperm female gametophyte. The detailed transcriptional characterization of different stages during female gametophyte development has significantly helped to improve our understanding of cellular specification or cell-cell communication processes. Next-generation sequencing approaches-used increasingly for expression profiling-will now allow for comparative approaches that focus on agriculturally, ecologically or evolutionarily relevant aspects of plant reproduction.

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Additional indexing

Item Type:Journal Article, refereed, further contribution
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
07 Faculty of Science > Institute of Evolutionary Biology and Environmental Studies
07 Faculty of Science > Zurich-Basel Plant Science Center
Dewey Decimal Classification:570 Life sciences; biology
590 Animals (Zoology)
580 Plants (Botany)
Language:English
Date:2013
Deposited On:22 Feb 2013 14:02
Last Modified:07 Dec 2017 19:54
Publisher:Elsevier
ISSN:1369-5266
Publisher DOI:https://doi.org/10.1016/j.pbi.2012.12.001
PubMed ID:23276786

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