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Mapping of Indian bread wheat population, HS 2189 x Agra Local for adult plant leaf rust resistance, Lr34


Saharan, M S; Tiwari, R; Krattinger, S G; Keller, B; Priyamvada, A (2012). Mapping of Indian bread wheat population, HS 2189 x Agra Local for adult plant leaf rust resistance, Lr34. Indian Phytopathology, 65:128-132.

Abstract

To identify and map quantitative trait loci (QTLs) for leaf rust resistance, 128 single seed descent lines of an ‘HD2189’בAgra Local’ F6 population were analyzed with molecular markers. Percent leaf area infected and the response to infection recorded in two field trials in 2006-07 at Karnal (India) were transformed to the area under the disease progress curve (AUDPC). Above 128 recombinant inbred lines along with parental lines were analysed with six polymorphic simple sequence repeats (SSRs) markers (GWM1220, XWM1, SWM5, SWM8, SWM10 & WMC463) and one sequence tagged sites (STS) marker, csLV34. A genetic linkage map based on above markers data was established. The region containing Lr34 was flanked by XSWM8 (proximal) and XWMC463 (distal) and spanned 68.7 CM in ‘HD 2189’בAgra Local’ population. Marker SWM10 was closely located to Lr34 in present population. By using composite interval mapping and LOD >4.4, QTL analysis of the linkage group representing chromosome 7DS in the HD 2189×Agra Local population defined a QTL for leaf rust resistance in the interval XGWM1220 - XSWM10 which accounted for 14.8 % of observed phenotypic variation.

Abstract

To identify and map quantitative trait loci (QTLs) for leaf rust resistance, 128 single seed descent lines of an ‘HD2189’בAgra Local’ F6 population were analyzed with molecular markers. Percent leaf area infected and the response to infection recorded in two field trials in 2006-07 at Karnal (India) were transformed to the area under the disease progress curve (AUDPC). Above 128 recombinant inbred lines along with parental lines were analysed with six polymorphic simple sequence repeats (SSRs) markers (GWM1220, XWM1, SWM5, SWM8, SWM10 & WMC463) and one sequence tagged sites (STS) marker, csLV34. A genetic linkage map based on above markers data was established. The region containing Lr34 was flanked by XSWM8 (proximal) and XWMC463 (distal) and spanned 68.7 CM in ‘HD 2189’בAgra Local’ population. Marker SWM10 was closely located to Lr34 in present population. By using composite interval mapping and LOD >4.4, QTL analysis of the linkage group representing chromosome 7DS in the HD 2189×Agra Local population defined a QTL for leaf rust resistance in the interval XGWM1220 - XSWM10 which accounted for 14.8 % of observed phenotypic variation.

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Additional indexing

Item Type:Journal Article, not refereed, original work
Communities & Collections:07 Faculty of Science > Department of Plant and Microbial Biology
Dewey Decimal Classification:580 Plants (Botany)
Language:English
Date:2012
Deposited On:28 Feb 2013 16:57
Last Modified:05 Apr 2016 16:34
Publisher:Indian Phytopathological Society
ISSN:0367-973X
Official URL:http://epubs.icar.org.in/ejournal/index.php/IPPJ/article/view/18573

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