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Development and validation of a PulseNet standardized protocol for sub-typing isolates of Cronobacter species


Brengi, S; O’Brien, S B; Pichel, M; Iversen, C; Arduino, M; Binsztein, N; Jensen, B; Pagotto, F; Ribot, E; Stephan, R; Cernela, N; Cooper, K; Fanning, S (2012). Development and validation of a PulseNet standardized protocol for sub-typing isolates of Cronobacter species. Foodborne Pathogens and Disease, 9(9):861-867.

Abstract

Cronobacter (formerly known as Enterobacter sakazakii) is a genus comprising seven species regarded as opportunistic pathogens that can be found in a wide variety of environments and foods, including powdered infant formula (PIF). Cronobacter sakazakii, the major species of this genus, has been epidemiologically linked to cases of bacteremia, meningitis in neonates, and necrotizing enterocolitis, and contaminated PIF has been identified as an important source of infection. Robust and reproducible subtyping methods are required to aid in the detection and investigation, of foodborne outbreaks. In this study, a pulsed-field gel electrophoresis (PFGE) protocol was developed and validated for subtyping Cronobacter species. It was derived from an existing modified PulseNet protocol, wherein XbaI and SpeI were the primary and secondary restriction enzymes used, generating an average of 14.7 and 20.3 bands, respectively. The PFGE method developed was both reproducible and discriminatory for subtyping Cronobacter species.

Abstract

Cronobacter (formerly known as Enterobacter sakazakii) is a genus comprising seven species regarded as opportunistic pathogens that can be found in a wide variety of environments and foods, including powdered infant formula (PIF). Cronobacter sakazakii, the major species of this genus, has been epidemiologically linked to cases of bacteremia, meningitis in neonates, and necrotizing enterocolitis, and contaminated PIF has been identified as an important source of infection. Robust and reproducible subtyping methods are required to aid in the detection and investigation, of foodborne outbreaks. In this study, a pulsed-field gel electrophoresis (PFGE) protocol was developed and validated for subtyping Cronobacter species. It was derived from an existing modified PulseNet protocol, wherein XbaI and SpeI were the primary and secondary restriction enzymes used, generating an average of 14.7 and 20.3 bands, respectively. The PFGE method developed was both reproducible and discriminatory for subtyping Cronobacter species.

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Additional indexing

Item Type:Journal Article, refereed, original work
Communities & Collections:05 Vetsuisse Faculty > Institute of Food Safety and Hygiene
Dewey Decimal Classification:570 Life sciences; biology
610 Medicine & health
Language:English
Date:2012
Deposited On:12 Mar 2013 08:30
Last Modified:07 Dec 2017 20:23
Publisher:Mary Ann Liebert
Additional Information:This is a copy of an article published in FOODBORNE PATHOGENS AND DISEASE © 2012 Mary Ann Liebert, Inc.; FOODBORNE PATHOGENS AND DISEASE is available online at: http://online.liebertpub.com.
Free access at:Publisher DOI. An embargo period may apply.
Publisher DOI:https://doi.org/10.1089/fpd.2012.1161

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